PMID: 7540902Feb 1, 1995Paper

Kinetic comparison of peptide: N-glycosidases F and A reveals several differences in substrate specificity

Glycoconjugate Journal
F AltmannC Weber

Abstract

The initial velocities of hydrolysis of nineteen glycopeptides by peptide: N-glycosidase F and A were determined. Substrates were prepared from bovine fetuin, hen ovalbumin, pineapple stem bromelain, bovine fibrin and taka-amylase. From these glycopeptides, several variants with regard to peptide and carbohydrate structure were prepared and derivatized with dabsyl chloride, dansyl chloride or activated resorufin. Tyrosine containing glycopeptides were also used without an additional chromophore. Enzymatic hydrolysis of glycopeptides was quantified by narrow bore, reversed phase HPLC with turnaround cycle times of down to 6 min, but usually 15 min. KM values ranging from 30 to 64 microM and from 4 to 36 microM were found for N-glycosidase F and A, respectively. Relative velocities of hydrolysis of the different substrates by each enzyme varied considerably. Little, if any, similarity of the performance of N-glycosidase F and A with the different substrates was observed. The minimal carbohydrate structure released by peptide: N-glycosidase F was a di-N-acetylchitobiose. N-glycosidase A could release even a single N-acetylglucosamine, albeit 3000 times slower than a di-N-acetylchitobiose or larger glycans. In general the structure...Continue Reading

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Citations

Nov 6, 2009·Glycoconjugate Journal·Elizabeth Higgins
Nov 1, 2001·Biochimica Et Biophysica Acta·P E Van den SteenP M Rudd
Mar 20, 2004·European Journal of Biochemistry·Martin GutterniggErika Staudacher
Sep 17, 2013·Applied Microbiology and Biotechnology·Martin DragositsDubravko Rendic
Feb 10, 2009·Phytochemistry·Renaud LéonardFriedrich Altmann
Nov 10, 2015·Journal of Agricultural and Food Chemistry·Ya M DuLi Liu
Nov 16, 2010·Molecular and Biochemical Parasitology·Amornrat GeadkaewRudi Grams
Mar 3, 2009·Biochemical and Biophysical Research Communications·Nobutake AkiyamaSaburo Saito
Mar 11, 2016·Biology of Reproduction·Ana Izabel Silva Balbin VillaverdeMark A Baker
Apr 21, 2017·International Journal of Molecular Sciences·Sercan KaravDaniela Barile
Jun 28, 2016·Angewandte Chemie·Yuntao ZhuXing Chen
Oct 6, 1997·The Journal of Biological Chemistry·N Ftouhi-PaquinT H Plummer
May 25, 2007·Molecular & Cellular Proteomics : MCP·Yoko KitaShin-Ichiro Nishimura
Apr 17, 2018·Bioscience, Biotechnology, and Biochemistry·Ryota UemuraYoshinobu Kimura
Dec 12, 2017·Mass Spectrometry Reviews·Qiwei ZhangJianjun Li
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Nov 10, 2006·The Journal of Biological Chemistry·Hidefumi ShinoharaYoshikatsu Matsubayashi
Dec 14, 2004·The Journal of Biological Chemistry·Renaud LeonardFriedrich Altmann
Jan 5, 2018·The Journal of Microbiology·Eun Jung ThakHyun Ah Kang

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