PMID: 6111340Dec 4, 1980Paper

Kinetic effects of the concentration-dependent stimulation of soluble guanylate cyclase from rat lung

Biochimica Et Biophysica Acta
M A LiebelA A White

Abstract

Soluble guanylate cyclase (GTP pyrophosphate-lyase (cyclizing), EC 4.6.1.2) from rat lung demonstrated concentration-dependent stimulation, that is, an increase in specific activity with increasing enzyme (protein) concentration. This phenomenon persisted through several steps of enzyme purification and was apparently due to the presence of a macromolecular activator, similar in size to the enzyme. Treatment of partially purified enzyme with N-ethylmaleimide destroyed catalytic activity, but did not effect the ability of the preparation to stimulate activity. Kinetic analysis demonstrated that the stimulation was due to an increased V value with no change in the apparent Km value for MnGTP. Stimulation occurred without a time lag, the activator apparently interacting reversibly with the enzyme to increase catalytic capability. Some nonionic detergents of the Triton series inhibited enzyme activity by decreasing the V value, with no change in the Km value, and also decreased concentration-dependent stimulation. However, the two phenomena were not directly related. While the physiological significance of the activator is unclear, its presence affects estimations of recovery during enzyme purification, V determinations, and determ...Continue Reading

References

Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W P ArnoldF Murad
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H Hidaka, T Asano
Apr 1, 1978·Analytical Biochemistry·A A White, D B Karr
Jan 1, 1974·Methods in Enzymology·A A White, T V Zenser
Jan 1, 1969·Biochimica Et Biophysica Acta·A A White, G D Aurbach
Feb 24, 1970·Biochimica Et Biophysica Acta·L S BradhamM Sims
May 1, 1973·Analytical Biochemistry·P W Holloway
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J M BraughlerF Murad
Sep 25, 2008·The Journal of Clinical Endocrinology and Metabolism·Jessica E MatthewsUNKNOWN Albiglutide Study Group
Oct 16, 2008·Environmental Science & Technology·Heather M StapletonThomas F Webster

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Citations

Feb 11, 1982·Biochemical and Biophysical Research Communications·M A Liebel, A A White

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