Kinetic evidence for unique regulation of GLUT4 trafficking by insulin and AMP-activated protein kinase activators in L6 myotubes.

The Journal of Biological Chemistry
Daniel J FazakerleyAdelle C F Coster

Abstract

In L6 myotubes, redistribution of a hemagglutinin (HA) epitope-tagged GLUT4 (HA-GLUT4) to the cell surface occurs rapidly in response to insulin stimulation and AMP-activated protein kinase (AMPK) activation. We have examined whether these separate signaling pathways have a convergent mechanism that leads to GLUT4 mobilization and to changes in GLUT4 recycling. HA antibody uptake on GLUT4 in the basal steady state reached a final equilibrium level that was only 81% of the insulin-stimulated level. AMPK activators (5-aminoimidazole-4-carboxyamide ribonucleoside (AICAR) and A-769662) led to a similar level of antibody uptake to that found in insulin-stimulated cells. However, the combined responses to insulin stimulation and AMPK activation led to an antibody uptake level of approximately 20% above the insulin level. Increases in antibody uptake due to insulin, but not AICAR or A-769662, treatment were reduced by both wortmannin and Akt inhibitor. The GLUT4 internalization rate constant in the basal steady state was very rapid (0.43 min(-1)) and was decreased during the steady-state responses to insulin (0.18 min(-1)), AICAR (0.16 min(-1)), and A-769662 (0.24 min(-1)). This study has revealed a nonconvergent mobilization of GLUT4...Continue Reading

References

Jan 1, 1992·The American Journal of Physiology·K J RodnickJ O Holloszy
May 1, 1991·Journal of Applied Physiology·E J HenriksenJ O Holloszy
Oct 1, 1994·Journal of Applied Physiology·J GaoJ O Holloszy
Apr 13, 1999·The American Journal of Physiology·Y PengR J Alpern
Dec 12, 2001·American Journal of Physiology. Endocrinology and Metabolism·Jonathan S FisherLorraine A Nolte
May 15, 2002·American Journal of Physiology. Endocrinology and Metabolism·Jonathan S FisherJohn O Holloszy
Nov 5, 2003·Molecular Biology of the Cell·Ola KarylowskiTimothy E McGraw
Jan 19, 2006·American Journal of Physiology. Endocrinology and Metabolism·Nadeeja WijesekaraAmira Klip
Oct 31, 2007·The Journal of Biological Chemistry·Joseph M MurettaCynthia Corley Mastick
Nov 13, 2007·The Biochemical Journal·Shuai ChenCarol Mackintosh
Feb 4, 2009·Diabetes·Håkan K R KarlssonGeoffrey D Holman

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Citations

Jan 22, 2011·The Journal of Biological Chemistry·Estifanos N HabtemichaelCynthia Corley Mastick
Mar 23, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Marta A PaivaMihaela M Mocanu
Mar 22, 2012·Endocrinology·Kirk M HabeggerJeffrey S Elmendorf
Jan 17, 2012·Journal of Cell Science·Jacqueline StöckliDavid E James
Feb 15, 2014·Cellular and Molecular Life Sciences : CMLS·Mirko TantimonacoMauro Maccarrone
Aug 9, 2013·Bioscience, Biotechnology, and Biochemistry·Eun-Hei LeeBae-Hwan Kim
Mar 16, 2011·Experimental Cell Research·Nicole M I NivillacImogen R Coe
Nov 8, 2015·European Journal of Pharmacology·Chandni S ThakkarAsha A Kulkarni-Almeida
Mar 16, 2011·Traffic·Alexander F RowlandDavid E James
Feb 5, 2014·British Journal of Pharmacology·T A M AlmabroukS Kennedy
Aug 18, 2010·Communicative & Integrative Biology·Jacqueline StöckliDavid E James
Mar 17, 2011·Biochemistry·Kevin FoleyAmira Klip
Mar 31, 2018·The Journal of Biological Chemistry·Daniel J FazakerleyDavid E James
Jan 10, 2013·Clinical Science·Silvia BijlandIan P Salt
Aug 19, 2011·Pflügers Archiv : European journal of physiology·Deborah L GraingerKatherine A Hinchliffe
Aug 1, 2013·Physiological Reviews·Erik A Richter, Mark Hargreaves
Jun 5, 2014·American Journal of Physiology. Endocrinology and Metabolism·Q LiW Niu
Oct 18, 2019·Traffic·Sadia RahmaniCostin N Antonescu
Jul 3, 2020·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Hiromi SatoKoichi Ueno
Feb 13, 2016·International Journal of Molecular Medicine·Jong-Hyeok HaYoosik Yoon
Jul 18, 2012·Current Opinion in Nephrology and Hypertension·Núria M Pastor-Soler, Kenneth R Hallows
Oct 22, 2015·American Journal of Physiology. Endocrinology and Metabolism·Gregory D Cartee
Apr 30, 2014·The Journal of Biological Chemistry·Paul Duffield BrewerAdelle C F Coster
Nov 22, 2016·Molecular and Cellular Biochemistry·Kalyana C NandipatiDevendra K Agrawal
Mar 8, 2020·Nature Reviews. Molecular Cell Biology·Pearl LeeM Celeste Simon
Nov 12, 2020·Molecular Biology of the Cell·Alexandria BrumfieldTimothy E McGraw
Oct 9, 2020·The FEBS Journal·Katharina HuberLucia C Leal-Esteban
May 8, 2021·Frontiers in Endocrinology·Man Mohan ShresthaWeiping Han

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