Kinetic folding and cis/trans prolyl isomerization of staphylococcal nuclease. A study by stopped-flow absorption, stopped-flow circular dichroism, and molecular dynamics simulations

Biochemistry
T IkuraK Kuwajima

Abstract

We studied the urea-induced unfolding transition of staphylococcal nuclease (SNase) and its five proline mutants (P47A, P47T, P117G, P47T/P117G, and P47A/P117G) [corrected] by peptide and aromatic circular dichroism and aromatic absorption spectroscopy at equilibrium and the refolding-unfolding kinetics of the proteins by stopped-flow circular dichroism and stopped-flow absorption techniques. Recent studies have revealed that the cis/trans isomerizations about the Pro47 and Pro117 peptide bonds of SNase occur not only in the unfolded state but also in the native state. The mutational effects on the stability and the refolding-unfolding kinetics of SNase were, however, remarkably different between the two sites. The substitution of Ala or Thr for Pro47 neither changed the stability nor affected the refolding-unfolding kinetics of SNase, whereas the substitution of Gly for Pro117 increased the protein stability by 1.2 kcal/mol (pH 7.0 and 20 degrees C) and affected the kinetics. These results have been attributed to the high flexibility of the loop around Pro47, which has been revealed by molecular dynamics simulations of native SNase. Under every condition studied, cooperative refolding-unfolding kinetics of SNase were observed....Continue Reading

References

Nov 20, 1992·Journal of Molecular Biology·D P RaleighC M Dobson
Mar 5, 1992·Journal of Molecular Biology·T KiefhaberF X Schmid
Jan 1, 1987·Annual Review of Biophysics and Biophysical Chemistry·J A Schellman
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K Kuwajima
Feb 1, 1996·Current Opinion in Structural Biology·A D Miranker, C M Dobson
Jul 26, 1996·Journal of Molecular Biology·A T AlexandrescuM J Blommers
Jan 1, 1996·Folding & Design·R L Baldwin

❮ Previous
Next ❯

Citations

Dec 19, 2002·Biophysical Chemistry·Louise A Wallace, C Robert Matthews
Jun 4, 2013·Amino Acids·Pierrick CraveurJoseph Rebehmed
Jan 14, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Akihiro FukagawaMakio Tokunaga
Feb 14, 2006·Biochimica Et Biophysica Acta·Nikolai Smolin, Roland Winter
Sep 17, 2008·Protein Science : a Publication of the Protein Society·Byoung-Chul Lee, Wouter D Hoff
Aug 2, 2020·Proceedings of the National Academy of Sciences of the United States of America·Takuya MizukamiKosuke Maki
Dec 18, 2001·Journal of Molecular Biology·N Bhutani, J B Udgaonkar
Jan 10, 2002·Journal of Molecular Biology·Louise A Wallace, C Robert Matthews
May 19, 2004·Journal of Molecular Biology·Matthias WeiwadGunter Fischer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.