PMID: 2107875Mar 26, 1990Paper

Kinetic mechanism of beta-glucosidase from Trichoderma reesei QM 9414

Biochimica Et Biophysica Acta
P EstradaC Acebal

Abstract

beta-Glucosidase is a key enzyme in the hydrolysis of cellulose to D-glucose. beta-Glucosidase was purified from cultures of Trichoderma reesei QM 9414 grown on wheat straw as carbon source. The enzyme hydrolyzed cellobiose and aryl beta-glucosides. The double-reciprocal plots of initial velocity vs. substrate concentration showed substrate inhibition with cellobiose and salicin. However, when p-nitrophenyl beta-D-glucopyranoside was the substrate no inhibition was observed. The corresponding kinetic parameters were: K = 1.09 +/- 0.2 mM and V = 2.09 +/- 0.52 mumol.min-1.mg-1 for salicin; K = 1.22 +/- 0.3 mM and V = 1.14 +/- 0.21 mumol.min-1.mg-1 for cellobiose; K = 0.19 +/- 0.02 mM and V = 29.67 +/- 3.25 mumol.min-1.mg-1 for p-nitrophenyl beta-D-glucopyranoside. Studies of inhibition by products and by alternative product supported an Ordered Uni Bi mechanism for the reaction catalyzed by beta-glucosidase on p-nitrophenyl beta-D-glucopyranoside as substrate. Alternative substrates as salicin and cellobiose, a substrate analog such as maltose and a product analog such as fructose were competitive inhibitors in the p-nitrophenyl beta-D-glucopyranoside hydrolysis.

References

May 1, 1976·Applied and Environmental Microbiology·D Sternberg
Jan 1, 1979·Methods in Enzymology·F B Rudolph
Jul 1, 1977·Biotechnology and Bioengineering·C S GongG T Tsao
Oct 1, 1971·Biochimica Et Biophysica Acta·G M Umezurike
Feb 1, 1970·Journal of Bacteriology·B M Eberhart, R S Beck
Jan 1, 1983·Cancer Investigation·B J Druker, H T Wepsic

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Citations

Jul 1, 1995·Research in Microbiology·R MarascoM De Felice
May 29, 1999·Applied and Environmental Microbiology·D StollR A Warren
Apr 6, 2021·Journal of Chemical Information and Modeling·Anderson A GomesMaria de Lourdes B Magalhães
Oct 25, 1991·Biochimica Et Biophysica Acta·X L Li, R E Calza

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