Kinetic Mechanism of Thioflavin T Binding onto the Amyloid Fibril of Hen Egg White Lysozyme

Langmuir : the ACS Journal of Surfaces and Colloids
Zhe QinGang Ma

Abstract

Thioflavin T (ThT) is widely used as a fluorescent probe for amyloid fibril detection. Yet the exact kinetic mechanism of ThT binding onto amyloid fibril remains elusive. Previously reported kinetic studies using ThT-fluorescence-detected kinetic design suggested two completely different ThT-binding mechanisms. In one study, a multistep sequential binding mechanism onto a single ThT-binding site was suggested. In another study, a one-step parallel binding mechanism onto multiple ThT-binding sites was suggested. The discrepancy is likely due to the incapability of ThT-fluorescence-detected kinetic design to differentiate the two above-mentioned mechanisms. Considering the weakness of the ThT-fluorescence-detected approach, we investigated the ThT-binding mechanism onto the amyloid fibril of hen egg white lysozyme (HEWL) using a new approach, ThT-absorbance-detected kinetic design. Our new results suggest that ThT binds to HEWL fibril through the one-step parallel binding mechanism. We hope our work can offer some new insights into the interactions between dye molecules and amyloid fibrils.

References

Nov 14, 1997·Journal of Molecular Biology·M SundeC C Blake
Oct 3, 1999·Methods in Enzymology·W E KlunkR P Mason
Jul 1, 1965·American Journal of Clinical Pathology·D R ROGERS
Jul 9, 2004·Journal of Molecular Biology·Erica FrareAngelo Fontana
Jul 13, 2004·Biomacromolecules·Brian A VernagliaEliana D Clark
Oct 19, 2004·Biophysical Journal·Luben N Arnaudov, Renko de Vries
Jan 5, 2005·Journal of Structural Biology·M R H KrebsA M Donald
Aug 30, 2005·Journal of Structural Biology·Ritu KhuranaShashi Singh
Sep 13, 2005·Structure·Jimin WangLynne Regan
Jun 8, 2006·Annual Review of Biochemistry·Fabrizio Chiti, Christopher M Dobson
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Neil FergusonAlan R Fersht
Dec 28, 2006·Advances in Protein Chemistry·Rebecca Nelson, David Eisenberg
Feb 10, 2007·Journal of Structural Biology·Minna GroenningFlemming S Jørgensen
Feb 20, 2007·Journal of Proteome Research·Alexander A MaskevichKonstantin K Turoverov
May 1, 2007·Nature·Michael R SawayaDavid Eisenberg
Apr 15, 2008·Journal of Structural Biology·Raimon SabatéSven J Saupe
Apr 16, 2009·The Journal of Physical Chemistry. B·Vitali I StsiapuraKonstantin K Turoverov
Aug 12, 2009·The Journal of Physical Chemistry. B·Catherine C Kitts, David A Vanden Bout
Sep 29, 2009·Journal of Molecular Biology·Thomas R JahnLouise C Serpell
Oct 6, 2009·Journal of Molecular Biology·Chun WuJoan-Emma Shea
Jan 26, 2010·The Journal of Physical Chemistry. B·Prabhat K SinghSukhendu Nath
Feb 4, 2010·Chemical Communications : Chem Comm·Cristina Rodríguez-RodríguezPilar González-Duarte
Apr 20, 2010·Biochimica Et Biophysica Acta·Matthew Biancalana, Shohei Koide
Apr 5, 2011·Chemical Biology & Drug Design·Ashley A Reinke, Jason E Gestwicki
Aug 26, 2011·The Journal of Physical Chemistry. B·Anna I SulatskayaKonstantin K Turoverov
Jan 25, 2012·The Journal of Physical Chemistry. B·Anna I SulatskayaKonstantin K Turoverov
Jun 29, 2012·Accounts of Chemical Research·Nadav AmdurskyDan Huppert
May 21, 2013·Chemical Communications : Chem Comm·Raimon SabateSalvador Ventura
Aug 8, 2015·Chemical Communications : Chem Comm·Prabhat K SinghSukhendu Nath

❮ Previous
Next ❯

Citations

Feb 12, 2021·Chembiochem : a European Journal of Chemical Biology·Megan A RochaRachel W Martin
Mar 2, 2021·The Analyst·Catarina S H JesusCarlos Serpa
Oct 4, 2019·The Journal of Physical Chemistry. B·Mantas Ziaunys, Vytautas Smirnovas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Related Papers

Nihon Hifuka Gakkai zasshi. The Japanese journal of dermatology
N IshiiK Okuda
Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
Valerie A IvancicNoel D Lazo
International Journal of Biological Macromolecules
Sajad ShariatiziMohsen Nemat-Gorgani
© 2022 Meta ULC. All rights reserved