Kinetic model of thermophilic L-lactate fermentation by Bacillus coagulans combined with real-time PCR quantification

Water Research
Taira HidakaHiroshi Tsuno

Abstract

A simple L-lactate fermentation of organic wastes at pH 5.5 and 55 degrees C under nonsterile conditions using Bacillus coagulans can be suitable for L-lactate fermentation of garbage. A mathematical model that simulated the lactate fermentation characteristics of B. coagulans was developed by focusing on the inhibitory effects of substrate, lactate (product) and NaCl, and bacterial growth. Basic fermentation experiments were performed using simple substrates to derive fundamental parameters of growth rate and inhibition effects. The model was then applied to fermentations using simple substrates and artificial kitchen garbage in order to verify its applicability. Microbial concentration, a key state variable of the model was measured using both real-time polymerase chain reaction (PCR) and traditional methods. The results of these methods were compared for experimental cases in which only soluble substrates were used. B. coagulans concentrations were suitably measured using real-time PCR, even when traditional measurement methods for microbial concentrations cannot be used. The results indicate that the developed model and biomass measurement can be used to evaluate lactate fermentations using both simple and complex substrate...Continue Reading

References

Dec 6, 2005·Biotechnology Letters·Michal RosenbergKatarína Malátová
Sep 23, 2008·Journal of Bioscience and Bioengineering·Jihoon CheonHiroshi Tsuno
Feb 28, 2009·Environmental Science & Technology·Joo H HaHiroshi Tsuno

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Citations

Apr 9, 2016·Water Research·Mi-Sun KimDong-Hoon Kim
Sep 10, 2013·Bioresource Technology·Santiago García-GenJorge Rodríguez
Oct 7, 2016·Applied Microbiology and Biotechnology·Gyucheol ChoiChangsoo Lee

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