Kinetic peculiarities of human tissue kallikrein: 1--substrate activation in the catalyzed hydrolysis of H-D-valyl-L-leucyl-L-arginine 4-nitroanilide and H-D-valyl-L-leucyl-L-lysine 4-nitroanilide; 2--substrate inhibition in the catalyzed hydrolysis of N alpha-p-tosyl-L-arginine methyl ester

Archives of Biochemistry and Biophysics
Marinez O SousaAmintas F S Figueiredo

Abstract

Hydrolysis of D-valyl-L-leucyl-L-lysine 4-nitroanilide (1), D-valyl-L-leucyl-L-arginine 4-nitroanilide (2), and N alpha-p-tosyl-L-arginine methyl ester (3) by human tissue kallikrein was studied throughout a wide range of substrate concentrations. At low substrate concentrations, the hydrolysis followed Michaelis-Menten kinetics but, at higher substrate concentrations, a deviation from Michaelis-Menten behavior was observed. With the nitroanilides, a significant increase in hydrolysis rates was observed, while with the ester, a significant decrease in hydrolysis rates was observed. The results for substrates (1) and (3) can be accounted for by a model based on the hypothesis that a second substrate molecule binds to the ES complex to produce a more active or an inactive SES complex. The deviation observed for substrate (2) can be explained as a bimolecular reaction between the enzyme-substrate complex and a free substrate molecule.

References

Jul 15, 1988·The Biochemical Journal·R J MacDonaldE G Erdös
May 1, 1988·Biological Chemistry Hoppe-Seyler·E P GiustiE S Prado
May 1, 1988·Biological Chemistry Hoppe-Seyler·M S Araújo-VielE S Prado
Oct 8, 1974·Biochemistry·E SilvaM Mares-Guia
Jul 2, 1973·European Journal of Biochemistry·F FiedlerE Werle
Oct 23, 1970·Biochemical and Biophysical Research Communications·H Nakata, S I Ishii
Jul 1, 1980·Archives of Biochemistry and Biophysics·C KettnerE Shaw
May 11, 1982·Biochemistry·E AntoniniM Guarneri
Jan 1, 1981·Methods in Enzymology·R Geiger, H Fritz
Mar 1, 1980·Archives of Biochemistry and Biophysics·N M Magalhães-RochaM Mares-Guia
Jul 1, 1980·Chemico-biological Interactions·F A BelandF E Evans
Jan 11, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M O SousaA F Figueiredo
Nov 1, 1961·Archives of Biochemistry and Biophysics·B F ERLANGERW COHEN
Jul 1, 1963·Biochemistry·C G TROWBRIDGEM LASKOWSKI

❮ Previous
Next ❯

Citations

Jul 30, 2010·Parasitology Research·Raquel Elisa da Silva-LópezSalvatore Giovanni de Simone
Nov 24, 2004·Journal of Enzyme Inhibition and Medicinal Chemistry·Marinez Oliveira De SousaAmintas Fabiano De Souza Figueiredo
Jul 1, 2008·Molecular and Cellular Biochemistry·Rajdeep ChoudhuryTapati Chakraborti
Sep 15, 2004·Experimental Parasitology·Raquel Elisa da Silva-Lopez, Salvatore Giovanni-De-Simone
Jul 5, 2008·Acta Tropica·Raquel Elisa da Silva-LopezSalvatore Giovanni-De-Simone

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.