Kinetic process of beta-amyloid formation via membrane binding.

Biophysical Journal
Yen SunHuey W Huang

Abstract

Recently we have studied thermodynamics of membrane-mediated beta-amyloid formation in equilibrium experiments using penetratin-lipid mixtures. The results showed that penetratin bound to the membrane interface in the alpha-helical conformation when the peptide/lipid (P/L) ratios were below a lipid-dependent critical value P/L*. When P/L reached P/L*, small beta-aggregates emerged, which served as the nuclei for large beta-aggregates. Here we studied the corresponding kinetic process to understand the potential barriers for the membrane-mediated beta-amyloid formation. We performed kinetic experiments using giant unilamellar vesicles made of 7:3 DOPC/DOPG. The observed time behavior of individual giant unilamellar vesicles, although complex, exhibited the physical effects seen in equilibrium experiments. Most interestingly, a potential barrier appeared to block penetratin from translocating across the bilayer. As a result, the kinetic value for the critical threshold P/L* is roughly one-half of the value measured in equilibrium where peptides bind symmetrically on both sides of lipid bilayers. We also investigated the similarity and differences between the charged and neutral lipids in their interactions with penetratin. We rea...Continue Reading

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Citations

Jun 21, 2011·Biochemistry·Keisuke IkedaKatsumi Matsuzaki
Aug 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ming-Tao LeeHuey W Huang
Nov 10, 2013·Advances in Colloid and Interface Science·Annalisa ReliniAlessandra Gliozzi
Nov 20, 2014·The Journal of Biological Chemistry·Mayu S TerakawaYuji Goto
Dec 14, 2017·Quarterly Reviews of Biophysics·Huey W Huang, Nicholas E Charron
Dec 15, 2018·Biochimica Et Biophysica Acta. Biomembranes·William T Heller, Piotr A Zolnierczuk
Aug 6, 2014·Biochemistry·Yen-Fei ChenHuey W Huang
Aug 29, 2018·Biochemistry·Ming-Tao LeeHuey W Huang
Aug 30, 2019·The Journal of Physical Chemistry. B·Lucas Rodrigues de MelloEmerson Rodrigo da Silva

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