Kinetic profiling of in vivo lung cellular inflammatory responses to mechanical ventilation.

American Journal of Physiology. Lung Cellular and Molecular Physiology
Samantha J WoodsMichael R Wilson

Abstract

Mechanical ventilation, through overdistension of the lung, induces substantial inflammation that is thought to increase mortality among critically ill patients. The mechanotransduction processes involved in converting lung distension into inflammation during this ventilator-induced lung injury (VILI) remain unclear, although many cell types have been shown to be involved in its pathogenesis. This study aimed to identify the profile of in vivo lung cellular activation that occurs during the initiation of VILI. This was achieved using a flow cytometry-based method to quantify the phosphorylation of several markers (p38, ERK1/2, MAPK-activated protein kinase 2, and NF-κB) of inflammatory pathway activation within individual cell types. Anesthetized C57BL/6 mice were ventilated with low (7 ml/kg), intermediate (30 ml/kg), or high (40 ml/kg) tidal volumes for 1, 5, or 15 min followed by immediate fixing and processing of the lungs. Surprisingly, the pulmonary endothelium was the cell type most responsive to in vivo high-tidal-volume ventilation, demonstrating activation within just 1 min, followed by the alveolar epithelium. Alveolar macrophages were the slowest to respond, although they still demonstrated activation within 5 min. ...Continue Reading

References

Jul 1, 1992·Journal of Applied Physiology·Z FuJ B West
Jan 1, 1992·The Clinical Investigator·H Wirtz, M Schmidt
Jan 1, 1987·Journal of Applied Physiology·T KawanoA C Bryan
Mar 1, 1997·The Journal of Clinical Investigation·L TremblayA S Slutsky
Jan 28, 1998·American Journal of Respiratory and Critical Care Medicine·D Dreyfuss, G Saumon
Jun 25, 1998·American Journal of Respiratory and Critical Care Medicine·A S Slutsky, L N Tremblay
Dec 9, 1998·The American Journal of Physiology·J PuginJ C Chevrolet
Jul 17, 1999·The American Journal of Physiology·N E VlahakisR D Hubmayr
Feb 17, 2000·Cellular Signalling·K Ono, J Han
May 4, 2000·The New England Journal of Medicine·UNKNOWN Acute Respiratory Distress Syndrome NetworkArthur Wheeler
Jul 13, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·Y AshinoJ Bhattacharya
Nov 25, 2000·Journal of Applied Physiology·J B West
Mar 20, 2001·American Journal of Respiratory and Critical Care Medicine·H D HeldS Uhlig
Jul 2, 2002·American Journal of Respiratory Cell and Molecular Biology·Séverine Oudin, Jérôme Pugin
Oct 3, 2002·Nature Reviews. Immunology·Qiutang Li, Inder M Verma
Nov 5, 2002·The European Respiratory Journal·U UhligS Uhlig
Aug 30, 2003·American Journal of Respiratory and Critical Care Medicine·Wolfgang M KueblerStefan Uhlig
Apr 13, 2004·Journal of Applied Physiology·Shawn E Soutiere, Wayne Mitzner
Apr 7, 2005·Intensive Care Medicine·Luciano Gattinoni, Antonio Pesenti
Jun 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Silvia Schnyder-CandrianBruno Schnyder
Aug 6, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Peter O KrutzikGarry P Nolan
Apr 11, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ian B CoplandMartin Post
Apr 25, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Raja-Elie E AbdulnourPaul M Hassoun
Aug 1, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·James A FrankMichael A Matthay
Oct 14, 2006·American Journal of Respiratory and Critical Care Medicine·Pier Paolo TerragniV Marco Ranieri
Dec 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew J ThorleyTeresa D Tetley
May 1, 2007·Intensive Care Medicine·Fabien G EyalJames C Parker
Feb 17, 2009·American Journal of Respiratory and Critical Care Medicine·Michael R WilsonMasao Takata
Aug 18, 2009·American Journal of Respiratory Cell and Molecular Biology·Francis Boudreault, Daniel J Tschumperlin
Sep 15, 2009·Biochemical and Biophysical Research Communications·Mai IwakiYoshinori Hasegawa
Oct 21, 2009·Translational Research : the Journal of Laboratory and Clinical Medicine·Yung-Yang LiuLi-Fu Li
Mar 17, 2010·Intensive Care Medicine·Thomas JaecklinBrian P Kavanagh
Mar 8, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Szabolcs BertokMasao Takata
Apr 23, 2011·Journal of Applied Physiology·Anthony D DorrMasao Takata
Aug 30, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kieran P O'DeaMasao Takata
Oct 19, 2011·The European Respiratory Journal·Brijesh V PatelMasao Takata
Oct 29, 2011·American Journal of Respiratory Cell and Molecular Biology·Naoya FujinoMutsuo Yamaya

❮ Previous
Next ❯

Citations

Dec 9, 2015·Best Practice & Research. Clinical Anaesthesiology·Pedro Leme SilvaPatricia Rieken Macêdo Rocco
Jun 12, 2016·Thorax·Sanooj SoniMasao Takata
Dec 17, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ting WangStephen M Black
Nov 15, 2019·Expert Opinion on Therapeutic Targets·Sanooj SoniMichael R Wilson
Feb 19, 2020·Experimental Lung Research·Erika Tiemi KozimaFrank Silva Bezerra
Feb 19, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sanooj SoniMasao Takata
Mar 16, 2016·Current Opinion in Organ Transplantation·Stephen Chiu, Ankit Bharat
Aug 9, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Y S PrakashKurt R Stenmark
Mar 27, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Harald EhrhardtAnne Hilgendorff
Sep 27, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Esmond L ArrindellRandal K Buddington
Jun 9, 2016·Minerva pediatrica·Jinjie HuangJun Wu
Feb 11, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kai SuGuochang Hu
Apr 14, 2021·American Journal of Respiratory and Critical Care Medicine·Marissa W KohMichael R Wilson

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
bronchoalveolar
lavage

Software Mentioned

FlowJo
SPSS
Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.