PMID: 2118807Sep 7, 1990Paper

Kinetic properties of cation/H(+)-exchange: calcimycin (A23187)-mediated Ca2+/2H(+)-exchange on the bilayer lipid membrane

Biochimica Et Biophysica Acta
P PohlL S Yaguzhinsky

Abstract

The calcimycin (A23187)-mediated electrically silent flux of hydrogen ions coupled with a counter transport of calcium or magnesium ions was measured by the method of local pH changes recording in the unstirred layers near the planar bilayer lipid membrane (BLM). It was shown that: (1) the pH dependence of calcimycin-mediated Ca2+/2H+ exchange had a maximum at pH 7; (2) the apparent Michaelis constant for the alkali earth cations were higher at acidic pH than the corresponding values at alkaline pH; (3) the apparent Michaelis constant for calcium was similar to that for magnesium ions in agreement with calcimycine cation binding constants; (4) the ratio of calcium and magnesium fluxes was independent of pH in the pH range from 5 to 8. (5) the flux was proportional to the calcimycin concentration at pH greater than 6.3 and proportional to the square of the carrier concentration at pH less than 5; (6) the addition of calcium ion chelator EDTA increased the flux significantly. These data were discussed in terms of the model of cation/H(+)-exchange and it was concluded that the dissociation of the cation-carrier complex at the membrane/water interface played an important role in the process of calcimycine operation. The comparison ...Continue Reading

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Citations

Jan 6, 2010·Biosensors & Bioelectronics·Yinxi HuangPeng Chen
May 4, 2001·Biochimica Et Biophysica Acta·A Y AbramovO V Krasilnikov
Jan 28, 2003·Biophysical Journal·Steffen SerowyPeter Pohl
Aug 22, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Amal ArachicheJeanne Dachary-Prigent
Aug 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M S Anwer
Aug 15, 2020·Journal of Human Reproductive Sciences·Nining HandayaniArief Boediono
Jun 23, 2009·Journal of Agricultural and Food Chemistry·Jürgen Behr, Rudi F Vogel

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