PMID: 41600Oct 1, 1979

Kinetic properties of highly purified luciferase from fireflies Luciola mingrelica

Biokhimii︠a︡
N Iu Filippova, N N Ugarova

Abstract

Luciferase of the fireflies Luciola mingrelica was isolated from dried lanterns of fireflies and purified by chromatography on DEAE-Sephadex. The homogeneity of the preparation was determined by polyacrylamide gel disc electrophoresis. The molecular weight of the enzyme equal to 45000 was determined by disc electrophoresis in the presence of sodium dodecyl sulfate. The kinetic properties of the enzyme (V and Km for luciferin and ATP) within the pH-range of 7,0--8,5 were studied. The kinetic curves of the pH-dependences of log V and log Km for both substrates are bell-shaped, with a slope equal to 2. At pH optimum (7,7--7,9) the Km values for luciferin and ATP are 6,6 mkM and 0,3 mM, respectively. The properties of luciferase L. m. were compared to those of luciferase from fireflies Phophinus pyralis previously described in literature.

Related Concepts

Polyacrylamide Gels
Chromatography
Order Coleoptera
Electrophoresis, Disc
Luciferin
Luciola mingrelica
Sodium Dodecyl Sulfate
Sephadex
Pyralis
2-diethylaminoethanol

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