PMID: 11311853Apr 20, 2001Paper

Kinetic properties of human placental glucose-6-phosphate dehydrogenase

The International Journal of Biochemistry & Cell Biology
Nazmi OzerI Hamdi Oğüş

Abstract

The kinetic properties of placental glucose-6-phosphate dehydrogenase were studied, since this enzyme is expected to be an important component of the placental protection system. In this capacity it is also very important for the health of the fetus. The placental enzyme obeyed "Rapid Equilibrium Ordered Bi Bi" sequential kinetics with K(m) values of 40+/-8 microM for glucose-6-phosphate and 20+/-10 microM for NADP. Glucose-6-phosphate, 2-deoxyglucose-6-phosphate and galactose-6-phosphate were used with catalytic efficiencies (k(cat)/K(m)) of 7.4 x 10(6), 4.89 x 10(4) and 1.57 x 10(4) M(-1).s(-1), respectively. The K(m)app values for galactose-6-phosphate and for 2-deoxyglucose-6-phosphate were 10+/-2 and 0.87+/-0.06 mM. With galactose-6-phosphate as substrate, the same K(m) value for NADP as glucose-6-phosphate was obtained and it was independent of galactose-6-phosphate concentration. On the other hand, when 2-deoxyglucose-6-phosphate used as substrate, the K(m) for NADP decreased from 30+/-6 to 10+/-2 microM as the substrate concentration was increased from 0.3 to 1.5 mM. Deamino-NADP, but not NAD, was a coenzyme for placental glucose-6-phosphate dehydrogenase. The catalytic efficiencies of NADP and deamino-NADP (glucose-6-p...Continue Reading

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Citations

Feb 19, 2002·The International Journal of Biochemistry & Cell Biology·Nazmi OzerI Hamdi Ogüs
Dec 29, 2012·BMC Systems Biology·Gautham Vivek SridharanKyongbum Lee
Feb 16, 2016·Nature Methods·William D CameronJonathan V Rocheleau
Jul 15, 2006·Cell·Karim BensaadKaren H Vousden
Nov 5, 2016·Free Radical Research·Hung-Chi YangDaniel Tsun-Yee Chiu
Sep 11, 2019·Cells·Hung-Chi YangDaniel Tsun-Yee Chiu
Nov 8, 2019·Biochemical Society Transactions·Antonella Del-CorsoPiero Luigi Ipata
Aug 15, 2014·Toxicology and Industrial Health·Garima ShakyaRukkumani Rajagopalan
Mar 13, 2003·Biotechnology and Bioengineering·Carlota O Rangel-YaguiDaniel Blankschtein
Oct 5, 2010·The Journal of Physical Chemistry. B·Vladimir PrivmanEvgeny Katz
Jun 5, 2013·The Journal of Physical Chemistry. B·Vladimir PrivmanEvgeny Katz

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