PMID: 6403033Apr 21, 1983Paper

Kinetic properties of Na+/Ca2+ exchange in basolateral plasma membranes of rat small intestine

Biochimica Et Biophysica Acta
W E GhijsenC H van Os

Abstract

The presence of an Na+/Ca2+ exchange system in basolateral plasma membranes from rat small intestinal epithelium has been demonstrated by studying Na+ gradient-dependent Ca2+ uptake and the inhibition of ATP-dependent Ca2+ accumulation by Na+. The presence of 75 mM Na+ in the uptake solution reduces ATP-dependent Ca2+ transport by 45%, despite the fact that Na+ does not affect Ca2+-ATPase activity. Preincubation of the membrane vesicles with ouabain or monensin reduces the Na+ inhibition of ATP-dependent Ca2+ uptake to 20%, apparently by preventing accumulation of Na+ in the vesicles realized by the Na+-pump.l It was concluded that high intravesicular Na+ competes with Ca2+ from intravesicular Ca2+ binding sites. In the presence of ouabain, the inhibition of ATP-dependent Ca2+ transport shows a sigmoidal dependence on the Na+ concentration, suggesting cooperative interaction between counter transport of at least two sodium ions for one calcium ion. The apparent affinity for Na+ is between 15 and 20 mM. Uptake of Ca2+ in the absence of ATP can be enhanced by an Na+ gradient (Na+ inside greater than Na+ outside). This Na+ gradient-dependent Ca2+ uptake is further stimulated by an inside positive membrane potential but abolished b...Continue Reading

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Citations

Jan 1, 1985·The Journal of Membrane Biology·E BayerdörfferI Schulz
Mar 5, 1999·Biological Trace Element Research·G Leblondel, P Allain
Aug 23, 2011·The Journal of Physiological Sciences : JPS·Narattaphol CharoenphandhuJantarima Charoenphandhu
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