Kinetic properties of the GABAA receptor main conductance state of mouse spinal cord neurones in culture.

The Journal of Physiology
R L MacdonaldR E Twyman

Abstract

1. The kinetic properties of the main conductance state of gamma-aminobutyric acidA (GABA) receptor channels from somata of mouse spinal cord neurones in cell culture were investigated using patch clamp techniques. 2. Whole-cell GABA receptor currents increased in a concentration-dependent manner from 0.5 to 5 microM. 3. Single-channel currents were recorded with a main conductance state of 27.2 pS and a less frequent conductance state of 15.9 pS. The main conductance state opened singly and in bursts of several openings. 4. Mean open times of GABA receptor main conductance currents were increased and open-time frequency histograms were shifted to longer times as GABA concentration was increased from 0.5 to 5 microM. Three exponential functions were required to fit the histograms at all GABA concentrations, suggesting that the channel opened into at least three open states (O1, O2 and O3). The three functions had the same time constants (1.0 +/- 0.2, 3.7 +/- 0.4 and 11.3 +/- 0.5 ms; mean +/- S.D.) at each concentration. The increase in long open times with concentration was due to a shift in relative frequency of occurrence of openings from the shortest (O1) to the two longest (O2 and O3) open states. 5. Closed-time distributio...Continue Reading

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