PMID: 77Nov 20, 1975

Kinetic studies and effects of anions on creatine phosphokinase from skeletal muscle of rhesus monkey (Macaca mulatta)

Biochimica Et Biophysica Acta
W R Chegwidden, D C Watts

Abstract

A purification procedure for creatine kinase (EC 2.7.3.2) from muscle of the monke35--170 muequiv H+/mg protein per min at 30 degrees C and a yield of approx. 0.5 g/kg muscle. Assuming equilibrium kinetics, synergistic binding of substrates at one catalytic site is found for both the forward and back reactions. Kinetic constants for the binding of each substrate to the free enzyme and the enzyme-second substrate complex are determined and are compared with those for the enzyme from other species. Inhibition by small anions is determined in the presence of different combinations of substrates and products. SO4(2-) inhibits by simple competitive inhibition and probably binds at the site of the transferrable phosphoryl group. Inhibition by NO3-, NO2-, SCN- and Cl- is more complex and these ions are suggested to mimic the transferrable phosphoryl group in a planar transition-state complex. These anions stabilize the dead-end complex, enzyme-creatine-MgADP, which lacks the transferable phosphoryl group. The effects of these anions on the dissociation constants of the enzyme-substrate complexes is reported and is in accord with the above hypothesis. The dead-end complex in the absence of anion does not protect the essential thiol gro...Continue Reading

References

Dec 31, 1960·Nature·B R RABIN, D C Watts

Citations

Jan 1, 1979·The International Journal of Biochemistry·S H Grossman, E Mollo
Jan 1, 1984·The International Journal of Biochemistry·W R Chegwidden, D C Watts
Jan 1, 1985·The International Journal of Biochemistry·W R ChegwiddenG G Penny
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·K A GrayD D Summers
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M S KatzR I Gregerman
Nov 13, 2004·Archives of Insect Biochemistry and Physiology·Ashli E Brown, S H Grossman
Aug 1, 1980·Biochemical Pharmacology·W R Chegwidden, D C Watts
Jun 19, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Brenda C HeldS H Grossman

Related Concepts

Acetic Acids
Striadyne
Metazoa
Anions
Anthropoidea
Macro-Creatine Kinase
Enzyme Activation
Hydrogen-Ion Concentration
Iodoacetamide
Macaca mulatta

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.

Related Papers

The International Journal of Biochemistry
W R Chegwidden, D C Watts
Biochemical and Biophysical Research Communications
M Martinez-Carrion, B Boettcher
© 2021 Meta ULC. All rights reserved