Kinetic studies and unstructured models of lymphocyte metabolism in fed-batch culture

Biotechnology and Bioengineering
G A TruskeyR W Swartz

Abstract

The growth of two lymphocyte cell lines, a hybridoma cell line and a human cutaneous T cell lymphoma (HuT78), was studied in fed-batch culture, and unstructured models of growth developed. A criteria was established to insure that the growth rate varied by less than a specified tolerance throughout the culture period. Glutamine and serum were growth-limiting nutrients for both cell lines with half-maximal growth rates at 0. 53 mM glutamine and 0. 55%(v/v) serum for the hybridoma cells and 0. 21 mM glutamine and 1. 5% serum for the HuT-78 cells. Over the range of glucose concentrations from 5. 5 mM to 28 mM, the specific growth rate of hybridoma cells was independent of glucose concentration, whereas glucose concentrations above 5. 5 mM inhibited HuT-78 growth. For both cell lines, the growth rate was significantly inhibited by the addition of ammonium, although the hybridoma cell line was more affected by ammonia than was the HuT-78 cell line. Growth of HuT-78 cells increased in the presence of interleukin-2. Unstructured models for the hybridoma cells were similar to other models presented in the literature. Applications of these models to adoptive immunotherapy are discussed.

References

Apr 1, 1978·Journal of Cellular Physiology·H R ZielkeM Cornblath
Jan 1, 1986·Annual Review of Immunology·S A Rosenberg, M T Lotze
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A Zetterberg, O Larsson
Jan 1, 1984·Annual Review of Immunology·K A Smith
Nov 1, 1981·The Journal of Experimental Medicine·J E GootenbergR C Gallo
Nov 1, 1962·Experimental Cell Research·G L TRITSCH, G E MOORE
Mar 1, 1986·Biotechnology and Bioengineering·J P Tharakan, P C Chau
Aug 5, 1988·Biotechnology and Bioengineering·M W GlackenA J Sinskey
Mar 1, 1989·Biotechnology and Bioengineering·M Dalili, D F Ollis

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Citations

Feb 20, 1992·Biotechnology and Bioengineering·S S OzturkB O Palsson
Jan 1, 1994·Cytotechnology·E Tziampazis, A Sambanis
May 15, 1996·Annals of the New York Academy of Sciences·R K BienerE D Gilles
Aug 26, 2006·Biotechnology and Bioengineering·Liangzhi Xie, Daniel I C Wang

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