Kinetic studies of 51Cr and DF32P labelled granulocytes

British Journal of Haematology
C DreschJ Bauchet

Abstract

In vivo kinetic studies of granulocytes labelled in vitro with 51Cr and DF32P were carried out in nine haematologically normal subjects by isolation of the cells in the blood samples by the Ficoll-Isopaque flotation method. 51Cr and 32P specific activity of blood samples made of 93-98% granulocytes was studied. Distribution between marginated and circulating granulocyte pools was identical for both labelled cells and the marginated pool was similar to the circulating pool, except that it was lower in one subject who had a previous splenectomy. The half-disappearance time (T 1/2) was 16.1+/-2.2 h for 51Cr-labelled and 5.4+/-2.1 hr for DF32P-labelled granulocytes. In one case of a normal subject who previously received multiple transfusion homologous 51Cr-labelled granulocytes had a T 1/2 of less than 1 h.

References

Sep 1, 1973·British Journal of Haematology·J L Spivak, S Perry
Aug 1, 1971·The Journal of Clinical Investigation·C R BishopJ W Athens
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation·C E Mogensen
Sep 1, 1968·The Journal of Experimental Medicine·R van Furth, Z A Cohn
Aug 1, 1968·Journal of Cellular Physiology·D M WhitelawH F Batho
Dec 1, 1965·Proceedings of the Society for Experimental Biology and Medicine·E KauderM M Wintrobe

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Citations

May 1, 1977·British Journal of Haematology·P C Vincent
Jan 1, 1983·British Journal of Haematology·N Colas-LinhartB Bok
May 21, 2011·Orphanet Journal of Rare Diseases·Jean DonadieuChristine Bellanné Chantelot
Jan 1, 1977·Transactions of the Royal Society of Tropical Medicine and Hygiene·S MusumeciA Fischer
Jan 16, 2007·Journal of Biopharmaceutical Statistics·Fredrik JonssonScott Marshall
Jul 1, 1977·Scandinavian Journal of Haematology·C DreschA Faille
Feb 24, 1984·Pharmaceutisch Weekblad. Scientific Edition·R Willemze
Feb 1, 1985·Scandinavian Journal of Haematology·A M PetersJ P Lavender
Feb 21, 2013·Cellular and Molecular Life Sciences : CMLS·Janesh PillayLeo Koenderman
Jul 1, 1979·Cell and Tissue Kinetics·A Hagenbeek, A C Martens
Jul 1, 1984·Seminars in Nuclear Medicine·J B Alavi, J Hansell

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