PMID: 37080May 2, 1979

Kinetic studies of the interaction between MS2 phage and F pilus of Escherichia coli

European Journal of Biochemistry
T Date

Abstract

The kinetics of the binding reaction of MS2 phage to free F pili, which were highly purified from Escherichia coli, has been studied using a membrane filter assay. The rate of dissociation (kd) of the MS2-phage--F-pilus complex is very slow and follows first-order kinetics with a half-life of 4.2 h at 30 degrees C in the standard buffer. The dissociation rate is rather insensitive to temperature, but becomes more rapid at high ionic strength or at basic pH. In a 0.25 M ionic strength buffer, the half-life of the complex is about 1.0 min. The rate of association is very fast and follows second-order kinetics with the rate constant for association (ka) being 8 x 10(7) M-1 s-1 at 30 degrees C in the standard buffer. The rate of association is almost insensitive to ionic strength but slightly sensitive to pH or temperature. Monovalent cations can also promote the binding reaction as well as divalent cations but the complex formed with monovalent cation is unstable. A study of the kinetics of dissociation suggests that there are two types of interaction between MS2 phage and F pilus; one is a strong interaction formed with divalent cations and the other is a weak one formed with monovalent cations. The physical nature of the bonds i...Continue Reading

References

Nov 25, 1975·Journal of Molecular Biology·M Schwartz
Jul 1, 1970·Virology·W ParanchychR D Bradley
Jul 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·L G Caro, M Schnös
May 14, 1968·Journal of Molecular Biology·J A Steitz
Jul 1, 1963·Journal of Molecular Biology·J H STRAUSS, R L SINSHEIMER
Sep 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·C C BRINTONJ CARNAHAN
Apr 23, 1965·Science·R C VALENTINE, M STRAND
Nov 28, 1966·Journal of Molecular Biology·J E Argetsinger, G N Gussin
May 20, 1940·The Journal of General Physiology·M Delbrück

Citations

Jul 25, 2012·Archives of Virology·Tomonori InomataAkiko Kashiwagi
Jun 15, 2011·ACS Applied Materials & Interfaces·Ying WangDavid G Whitten
Jul 18, 2019·Nature Communications·Ran MengJunjie Zhang

Related Concepts

Coliphages
Alkalescens-Dispar Group
Hydrogen-Ion Concentration
Mathematics
Osmolality

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