PMID: 7030310Feb 1, 1981Paper

Kinetic studies of the mechanism of pig kidney aldehyde reductase

The Biochemical Journal
F F Morpeth, F M Dickinson

Abstract

Initial-rate measurements were made of the oxidations of pyridine-3-methanol and glycerol by NADP+ and of the reduction of the corresponding aldehydes by NADPH catalysed by pig kidney aldehyde reductase. In addition, a brief survey of the specificity of the enzyme towards aldehyde substrates and its sensitivity to the inhibitors ethacrynic acid, sodium barbitone and warfarin was made. The detailed kinetic work indicates a compulsory mechanism for aldehyde reduction, with NADPH binding before aldehyde. For alcohol oxidation, however, it is necessary to postulate the formation of kinetically significant amounts of binary complexes of the type enzyme-alcohol to explain the results. Thus, for alcohol oxidation random-order addition of substrates may occur. Inhibition studies of the kinetics of aldehyde reduction in the presence of the corresponding alcohol product provide further evidence for the existence of enzyme-alcohol complexes. Finally, detailed kinetic studies were made of the inhibition of pyridine-3-aldehyde reduction by sodium barbitone. The mechanism of the inhibition is discussed.

Citations

Sep 1, 1981·European Journal of Biochemistry·A J Rivett, K F Tipton
Mar 15, 1990·Biochemical Pharmacology·A BhatnagarS K Srivastava
Nov 26, 1987·Biochimica Et Biophysica Acta·A BhatnagarS K Srivastava
Mar 1, 1988·Archives of Biochemistry and Biophysics·A BhatnagarS K Srivastava

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