PMID: 2321Feb 16, 1976

Kinetic studies on cytochrome c oxidase by combined epr and reflectance spectroscopy after rapid freezing

Biochimica Et Biophysica Acta
H BeinertC R Hartzell

Abstract

1. Techniques and experiments are described concerned with the millisecond kinetics of EPT-detectable changes brought about in cytochrome c oxidase by reduced cytochrome c and, after reduction with various agents, by reoxidation with O2 or ferricyanide. Some experiments in the presence of ligands are also reported. Light absorption was monitored by low-temperature reflectance spectroscopy. 2. In the rapid phase of reduction of cytochrome c oxidase by cytochrome c (less than 50 ms) approx. 0.5 electron equivalent per heme a is transferred mainly to the low-spin heme component of cytochrome c oxidase and partly to the EPR-detectable copper. In a slow phase (less than 1 s) the copper is reoxidized and high-spin ferric heme signals appear with a predominant rhombic component. Simultaneously the absorption band at 655 nm decreases and the Soret band at 444 nm appears between the split Soret band (442 and 447 nm) of reduced cytochrome a. 3. On reoxidation of reduced enzyme by oxygen all EPR and optical features are restored within 6 ms. On reoxidation by O2 in the presence of an excess of reduced cytochrome c, states can be observed where the low-spin heme and copper signals are largely absent but the absorption at 655 nm is maximal,...Continue Reading

References

Feb 16, 1976·Biochimica Et Biophysica Acta·C R Hartzell, H Beinert
Dec 5, 1970·Nature·E AntoniniB G Malmström
Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·C R HartzellH Beinert
Dec 19, 1974·Biochimica Et Biophysica Acta·C R Hartzell, H Beinert

Citations

Apr 1, 1981·Journal of Bioenergetics and Biomembranes·M Fry, D E Green
Apr 1, 2010·Journal of Bioenergetics and Biomembranes·Miyuki SakaguchiTakashi Ogura
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·Graham PalmerL E Vickery
Oct 24, 2007·Antioxidants & Redox Signaling·Ilya Belevich, Michael I Verkhovsky
Mar 8, 2011·Photomedicine and Laser Surgery·Brendan J Quirk, Harry T Whelan
Jul 1, 1976·FEBS Letters·L C Petersen, L E Andréasson
Jul 22, 1985·FEBS Letters·P Brzezinski, B G Malmström
Jul 28, 1980·FEBS Letters·R Boelens, R Wever
Mar 25, 1991·FEBS Letters·R MitchellP R Rich
Aug 8, 1978·Biochimica Et Biophysica Acta·S Rosén
Feb 16, 1976·Biochimica Et Biophysica Acta·C R Hartzell, H Beinert
Sep 23, 1977·Biochemical and Biophysical Research Communications·C H SeiterR A Perreault
Jan 1, 1988·Annals of the New York Academy of Sciences·J M WrigglesworthP Nicholls
Oct 12, 1977·Biochimica Et Biophysica Acta·H Beinert, R W Shaw
May 1, 1978·Archives of Biochemistry and Biophysics·M Erecińska, D F Wilson
Mar 12, 1981·Biochimica Et Biophysica Acta·B Karlsson, L E Andréasson
Jan 15, 1980·Biochemical and Biophysical Research Communications·J Keyhani, E Keyhani
Nov 9, 1976·Biochimica Et Biophysica Acta·P NichollsF B Hansen
Apr 26, 1995·Biochimica Et Biophysica Acta·O Einarsdóttir
Feb 15, 1985·Biochemical and Biophysical Research Communications·K Kariman, D S Burkhart
Dec 17, 2011·Biochimica Et Biophysica Acta·Takashi Ogura
Apr 22, 2004·Biochimica Et Biophysica Acta·Marian FabianGraham Palmer
Apr 22, 2004·Biochimica Et Biophysica Acta·Bryan SchmidtShelagh Ferguson-Miller
Jul 8, 1980·Biochimica Et Biophysica Acta·H BeinertC R Hartzell
Nov 5, 1986·Biochimica Et Biophysica Acta·A C GorrenR Wever
Feb 15, 1977·European Journal of Biochemistry·R WeverB F Van Gelker
Feb 10, 2004·The Journal of Biological Chemistry·Marian FabianGraham Palmer
Jan 1, 1988·Annals of the New York Academy of Sciences·B C Hill

Related Concepts

Anaerobiosis
Isocyanides
Cytochrome c Group
Cytochrome c Oxidase Subunit VIa
Electron Spin Resonance Spectroscopy
Ferricyanides
Melting
Hydrogen-Ion Concentration
Oxidation-Reduction
Protein Conformation

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