PMID: 4018075Jul 1, 1985

Kinetic studies on reduction of cytochromes P-450 and b5 by dithionite

European Journal of Biochemistry
D R DavydovA I Archakov

Abstract

The kinetics of reduction of cytochromes P-450 and b5 by dithionite had been studied in solution and in microsomal and proteoliposomal membranes by the stopped-flow technique. In all the cases studied the kinetic curves of reduction of cytochrome b5 obey first-order kinetics in relation to cytochrome with the rate constant about 14 s-1 at 10.6 mM dithionite. The kinetic curves of reduction of cytochrome P-450 fit an equation for the sum of two exponentials with the parameters varying from system to system. The simple first-order kinetics of cytochrome P-450 reduction had been observed in the presence of non-ionic detergent Triton N-101. The apparent biphasity of cytochrome P-450 reduction by dithionite should be the result asymmetric distribution of hemoprotein in microsomal and proteoliposomal membranes as well as in cytochrome oligomers in solution.

References

May 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J M McCordI Fridovich
May 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·L Spatz, P Strittmatter
Aug 1, 1981·Hoppe-Seyler's Zeitschrift für physiologische Chemie·I Knack, K H Röhm

Citations

Dec 2, 2008·Expert Opinion on Drug Metabolism & Toxicology·D R Davydov, James R Halpert
Mar 15, 2011·Expert Opinion on Drug Metabolism & Toxicology·D R Davydov
Dec 14, 2011·Pharmacology & Therapeutics·James R Reed, W L Backes
Apr 17, 2013·Biotechnology and Applied Biochemistry·D R DavydovJames R Halpert

Related Concepts

Cytochrome b Group
Cytochrome P-450 Oxygenase
Sodium Dithionite
Niosomes
Microsomes, Liver
Oxidation-Reduction
Proteolipids
Solutions
Sulfites
Thermodynamics

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