PMID: 3756185Oct 17, 1986Paper

Kinetic studies on turtle pancreatic ribonuclease: a comparative study of the base specificities of the B2 and P0 sites of bovine pancreatic ribonuclease A and turtle pancreatic ribonuclease

Biochimica Et Biophysica Acta
H KatohD Meinsma

Abstract

Kinetic constants for the transesterification of eight dinucleoside phosphates CpX and UpX by bovine and turtle pancreatic ribonuclease were determined. Both ribonucleases have a preference for purine nucleotides at the position X. However, bovine ribonuclease, like other mammalian ribonucleases, prefers 6-amino bases at this site, while turtle ribonuclease prefers 6-keto bases. This difference in specificity at the B2 site may be explained by the substitution of glutamic acid at position 111 by valine in turtle ribonuclease. These results have been confirmed by inhibition studies with the four nucleoside triphosphates. Inhibition studies with pT and pTp showed that a cationic binding group (P0) for the 5'-phosphate of the pyrimidine nucleotides bound at the primary B1 site is present in turtle ribonuclease, although lysine at position 66 in bovine ribonuclease is absent in turtle ribonuclease. However, the side chain of lysine 122 in turtle ribonuclease is probably located in the correct position to take over the role as cationic P0 site.

References

May 1, 1978·European Journal of Biochemistry·W GaastraJ J Beintema
Dec 2, 1985·European Journal of Biochemistry·J J BeintemaC Schüller
Jan 1, 1971·Archiv für die gesamte Virusforschung·Y Y TsilinskyD K Lvov
Nov 1, 1968·Journal of Biochemistry·M ImazawaT Ukita
Aug 1, 1953·The Biochemical Journal·M DIXON
May 4, 1962·Biochemical and Biophysical Research Communications·H WITZEL, E A BARNARD
Nov 1, 1965·The Biochemical Journal·W Gamble, L D Wright
Aug 25, 2007·Annals of Oncology : Official Journal of the European Society for Medical Oncology·S KakoM Kurokawa

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Citations

Feb 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ricardo ZamelRichard A Collins
Jul 13, 2000·Protein Science : a Publication of the Protein Society·L VitaglianoL Mazzarella
Jan 15, 1994·European Journal of Biochemistry·W ZhaoJ Hofsteenge
Apr 24, 2012·The EMBO Journal·Rachel E HaurwitzJennifer A Doudna
Apr 19, 2016·Quarterly Reviews of Biophysics·Michael KovermannMagnus Wolf-Watz
May 28, 2014·BioMed Research International·Takeo TatsutaKazuo Nitta
Feb 8, 2014·PloS One·Besnik Krasniqi, Jeremy S Lee
Nov 23, 2006·Journal of Biochemical and Biophysical Methods·Rakefet RosenfeldRuth Ben-Yakar Maya
Jun 14, 1988·Biochemistry·J J BeintemaD G Glitz
Jan 1, 1988·Progress in Biophysics and Molecular Biology·J J BeintemaA Carsana
Jan 30, 2002·Biochemistry·Wolfgang MaretBert L Vallee
Feb 24, 2006·Biochemistry·Evgenii L Kovrigin, J Patrick Loria
Apr 17, 2021·International Journal of Biological Macromolecules·Aman Prakash, Manish Kumar
May 15, 1992·Analytical Biochemistry·K M PostekR K Sandwick
May 24, 1994·Biochemistry·S B delCardayré, R T Raines
Feb 19, 2002·Chemical Reviews·Ronald T. Raines
Dec 17, 1996·Biochemistry·A A FedorovS C Almo
May 8, 2002·Biochemistry·Roger Cole, J Patrick Loria

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