PMID: 6407880Jan 1, 1983Paper

Kinetic study of the activation process of frog epidermis pro-tyrosinase by trypsin

The International Journal of Biochemistry
J D GalindoF García-Cánovas

Abstract

1. The rate of tyrosinase formation has been calculated by coupling the activatory process of frog epidermis pro-tyrosinase by trypsin to the oxidation of L-DOPA to dopachrome. Under certain conditions ([trypsin]/[pro-tyrosinase] greater than or equal to 300), the lag period of the coupled reactions, tau, is independent of trypsin concentration. 2. The specific rate constant of tyrosinase formation at different temperatures has been calculated, ranging from 0.025 sec-1, at 5 degrees C to 0.248 sec-1, at 30 degrees C. 3. Thermodynamic parameters of the activatory process (delta G not equal to = + 18.5 kcal/mol; delta H not equal to = + 14.8 kcal/mol; delta S not equal to = -12.4 e.u.; Ea = + 15.3 kcal/mol), have been determined by the study of the system at different temperatures. These values are characteristic for a normal chemical reaction. 4. From these kinetic data, the order of products formation in the proteolytic step, can be determined, active tyrosinase being the last product released.

References

Jan 1, 1979·General Pharmacology·J L IborraJ A Lozano
Apr 21, 1975·Biochemical and Biophysical Research Communications·R B MikkelsenE L Triplett
Apr 23, 1974·Biochemical and Biophysical Research Communications·M AshidaE Onishi
Sep 10, 1970·Biochemical and Biophysical Research Communications·J S McGuire
Sep 1, 1981·The Biochemical Journal·J L IborraJ A Lozano

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Citations

May 1, 1993·Bulletin of Mathematical Biology·B H HavsteenR Varón
Jan 1, 1983·The International Journal of Biochemistry·J D GalindoJ A Lozano
Jul 12, 2005·Biological Chemistry·Fernando Gandía-HerreroJosefa Escribano
Jun 10, 2006·Journal of Theoretical Biology·R VarónB H Havsteen
Jan 1, 1986·Bulletin of Mathematical Biology·R VarónF García Carmona
Aug 14, 1984·Biochimica Et Biophysica Acta·R PeñafielJ A Lozano
Oct 25, 1991·Biochimica Et Biophysica Acta·M García-MorenoH Rix-Matzen
May 7, 1988·Journal of Theoretical Biology·R VarónA Vázquez

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