PMID: 162261Jan 1, 1979

Kinetic study of the inhibition of myosin ATPase activity by ADP

Acta Biochimica Et Biophysica; Academiae Scientiarum Hungaricae
G S Kelemen

Abstract

The kinetic parameters of the inhibition of monovalent cation activated myosin ATPase by ADP were investigated. The inhibitor constant (KI) was 1.65 X 10(-4) M and the maximal velocity (V) was 1.28 mumol Pi/mg myosin/min in the presence of 0.3M Kcl at 20 degrees C. The dependence of 1/VO on inhibitor concentration and the pH dependence of KI and Km (i.e. pKi approximately equal to pKm) show that the inhibition has a pure competitive character. The results are supported by energetic parameters, too. The enthalpy of the formation of (EI) complex was calculated. Similar results were obtained also in the presence of Rb+ activated myosin ATPase and subfragment-I K+ ATPase.

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