Kinetic study on the irreversible thermal denaturation of Schistosoma japonicum glutathione S-transferase

Biochimica Et Biophysica Acta
Indalecio Quesada-SorianoLuis García-Fuentes

Abstract

The thermal unfolding pathway of the Schistosoma japonicum glutathione S-transferase (Sj26GST) was previously interpreted by applying equilibrium thermodynamics and a reversible two-state model (Kaplan et al., (1997) Protein Science, 6, 399-406), though weak support for this interpretation was provided. In our study, thermal denaturation of Sj26GST has been re-examined by differential scanning calorimetry in the pH range of 6.5-8.5 and in the presence of the substrate and S-hexylglutathione. Calorimetric traces were found to be irreversible and highly scan-rate dependent. Thermogram shapes, as well as their scan-rate dependence, can be globally explained by assuming that thermal denaturation takes place according to one irreversible step described by a first-order kinetic constant that changes with temperature, as given by an Arrhenius equation. On the basis of this model, values for the rate constant as a function of temperature and the activation energy have been determined. Data also indicate that binding of GSH or S-hexylglutathione just exert a very little stabilising effect on the dimeric structure of the molecule.

References

Jan 1, 1997·Chemical Research in Toxicology·R N Armstrong
Aug 8, 2001·European Journal of Biochemistry·E Ortiz-SalmerónL García-Fuentes
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·John D HayesIan R Jowsey
Aug 2, 2005·Biophysical Chemistry·Anna MichnikKatarzyna Michalik

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Citations

Mar 5, 2010·Biophysical Chemistry·Jose M Sanchez-Ruiz
Aug 19, 2008·Biochimica Et Biophysica Acta·Rita GuzziChristopher Dennison
Jul 1, 2008·Biochimica Et Biophysica Acta·Indalecio Quesada-SorianoLuis García-Fuentes
Sep 26, 2009·Protein Science : a Publication of the Protein Society·Indalecio Quesada-SorianoLuis García-Fuentes
Dec 1, 2012·International Journal of Biological Macromolecules·José Wilson P CarvalhoMarcel Tabak

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