PMID: 2507739Sep 1, 1989Paper

Kinetics and equilibria of cyanide binding to prostaglandin H synthase

Journal of Inorganic Biochemistry
I D MacDonald, H B Dunford

Abstract

Cyanide binding to prostaglandin H (PGH) synthase results in a spectral shift in the Soret region. This shift was exploited to determine equilibrium and kinetic parameters of the cyanide binding process. At pH 8.0, ionic strength 0.22 M, 4 degrees C, the cyanide dissociation constant, determined from equilibrium experiments, is (65 +/- 10) microM. The binding rate constant is (2.8 +/- 0.2) x 10(3) M-1 s-1, and the dissociation rate constant is zero within experimental error. Through a kinetic study of the binding process as a function of pH, from pH 3.96 to 8.00, it was possible to determine the pKa of a heme-linked acid group on the enzyme of 4.15 +/- 0.10 with citrate buffer. An apparent pKa of 4.75 +/- 0.03 was determined with acetate buffer; this different value is attributed to complexation of the enzyme with one of the components of the acetate buffer.

References

May 1, 1975·Canadian Journal of Biochemistry·D DolmanM D Thurlow
May 25, 1977·Biochimica Et Biophysica Acta·F J Van der OuderaaD A Van Dorp
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D L DeWitt, W L Smith
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M HambergB Samuelsson
Jun 30, 1980·Biochemical and Biophysical Research Communications·T Araiso, H B Dunford

❮ Previous
Next ❯

Citations

Mar 20, 1999·Biochimica Et Biophysica Acta·V Koshkin, H B Dunford
Apr 22, 2004·The Journal of Biological Chemistry·Wen LiuRichard J Kulmacz
May 1, 1996·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M Bakovic, H B Dunford
Nov 1, 1994·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M Bakovic, H B Dunford
Jul 26, 2007·The Journal of Biological Chemistry·Ilia G DenisovStephen G Sligar
Apr 16, 1998·The Journal of Biological Chemistry·V Koshkin, H B Dunford
Jan 26, 1996·The Journal of Biological Chemistry·M Bakovic, H B Dunford

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.