PMID: 8611408Apr 1, 1996Paper

Kinetics and localisation of PpIX fluorescence after topical and systemic ALA application, observed in skin and skin tumours of UVB-treated mice

British Journal of Cancer
N van der VeenW M Star

Abstract

In this study the kinetics and localisation of protoporphyrin IX (PpIX) fluorescence in skin and skin tumours were examined after topically (20% for 4h) or systemically (200 mg/kg,i.p.) administered 5-aminolaevulinic acid (ALA). As a model we used hairless mice with skin lesions (actinic keratoses and squamous cell carcinoma), which were induced by daily UVB irradiation. The epidermis of the skin surrounding the tumours (T) was altered (AS); owing to the UVB irradiation, the epidermis was thicker and less elastic. Therefore, non-UVB-irradiated mice were used to assess fluorescence of normal skin (NS). Light from a halogen lamp was used to excite at 500 +/- 20 nm and fluorescence was detected through a filter that passes light of 670 +/- 50 nm. Maximal fluorescence following i.p. ALA was observed 2 h post injection (p.i.) and was three times less than after topically applied ALA. Furthermore, after i.p. ALA a lower T selectively (T/NS) could be obtained than after topically applied ALA. Maximal fluorescence following topically applied ALA was achieved 6 h after the end of the 4 h application time. At that interval fluorescence of T was twice as high as directly after the application period. Furthermore, T selectivity (T/NS) afte...Continue Reading

Citations

Feb 28, 2003·Lasers in Surgery and Medicine·Sirintra CharoenbanpachonPetra Wilder-Smith
Sep 24, 2004·Photodermatology, Photoimmunology & Photomedicine·Norbert J NeumannPercy Lehmann
May 11, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Vivien SchachtChristoph Abels
Nov 30, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Henriëtte S de BruijnDominic J Robinson
Jun 19, 2008·Photochemistry and Photobiology·Bastiaan KruijtDominic J Robinson
Jul 3, 1997·International Journal of Cancer. Journal International Du Cancer·N Van der VeenW M Star
Oct 19, 2016·Lasers in Surgery and Medicine·Hyoseung ShinOhsang Kwon
Jan 8, 2003·Lasers in Surgery and Medicine·Arata EbiharaPetra Wilder-Smith
Jun 1, 2005·Plastic and Reconstructive Surgery·Cheng-Jen Chang, Petra Wilder-Smith
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Nov 30, 2019·Facial Plastic Surgery Clinics of North America·Lawrence S MoyStephanie Moy

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