Kinetics and mechanism of intercellular ice propagation in a micropatterned tissue construct

Biophysical Journal
Daniel Irimia, Jens O M Karlsson

Abstract

Understanding the effects of cell-cell interaction on intracellular ice formation (IIF) is required to design optimized protocols for cryopreservation of tissue. To determine the effects of cell-cell interactions during tissue freezing, without confounding effects from uncontrolled factors (such as time in culture, cell geometry, and cell-substrate interactions), HepG2 cells were cultured in pairs on glass coverslips micropatterned with polyethylene glycol disilane, such that each cell interacted with exactly one adjacent cell. Assuming the cell pair to be a finite state system, being either in an unfrozen state (no ice in either cell), a singlet state (IIF in one cell only), or a doublet state (IIF in both cells), the kinetics of state transitions were theoretically modeled and cryomicroscopically measured. The rate of intercellular ice propagation, estimated from the measured singlet state probability, increased in the first 24 h of culture and remained steady thereafter. In cell pairs cultured for 24 h and treated with the gap junction blocker 18beta-glycyrrhetinic acid before freezing, the intercellular ice propagation rate was lower than in untreated controls (p < 0.001), but significantly greater than zero (p < 0.0001). T...Continue Reading

References

Oct 13, 1992·Annals of the New York Academy of Sciences·M L YarmushR G Tompkins
Feb 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J H Kelly, G J Darlington
Jan 14, 1986·Biochemical and Biophysical Research Communications·J S DavidsonE H Harley
Dec 1, 1972·Cryobiology·L E McGannH E Frey
Feb 1, 1994·Biophysical Journal·K Muldrew, L E McGann
Feb 1, 1996·Biomaterials·J O Karlsson, M Toner
Feb 1, 1997·Journal of Biomedical Materials Research·S N BhatiaM Toner
May 30, 1997·Science·C S ChenD E Ingber
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·E C TozerJ C Loftus
Jul 22, 1999·Cryobiology·J P AckerL E McGann
May 5, 2001·Cryobiology·T B Darr, A Hubel
Oct 1, 1938·Plant Physiology·I H Stuckey, O F Curtis

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Citations

Oct 19, 2007·Annals of Biomedical Engineering·Saravana Kumar BalasubramanianJohn C Bischof
May 24, 2007·Tissue Engineering·Antonios G MikosGordana Vunjak-Novakovic
Jul 31, 2009·Journal of Biomechanical Engineering·Alptekin AksanJohn C Bischof
May 27, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Dawn M PedrottyNenad Bursac
Jul 8, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Shinsuke SekiPeter Mazur
Jan 18, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Shinsuke Seki, Peter Mazur
Feb 22, 2012·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·A StolzingS Sethe
Dec 2, 2008·Cryobiology·Shannon L Stott, Jens O M Karlsson
Dec 17, 2005·Biotechnology and Bioengineering·Mauris N De SilvaDavid J Odde
Jun 22, 2012·Biotechnology Progress·Rita MalpiquePaula M Alves
Dec 14, 2005·Cryobiology·Saravana Kumar BalasubramanianAllison Hubel
Oct 12, 2004·Biophysical Journal·Daniel Irimia, Jens O M Karlsson
Nov 12, 2013·Biophysical Journal·Janet A W Elliott
Feb 25, 2015·Cryobiology·Richelle C PrickettLocksley E McGann
May 14, 2011·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Charles J Hunt
Apr 3, 2004·Technology in Cancer Research & Treatment·Shannon L StottJens O M Karlsson
Feb 27, 2018·Tissue Engineering. Part C, Methods·Rui LiAllison Hubel
Nov 18, 2006·Physics in Medicine and Biology·A ZhangS Cheng
Nov 30, 2018·Biopreservation and Biobanking·Ariadna CorralRamón Risco
Sep 10, 2019·Biomacromolecules·Ruben M F TomásMatthew I Gibson
Nov 13, 2017·ACS Biomaterials Science & Engineering·Harishankar Natesan, John C Bischof

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