Kinetics and Optimization of Lipophilic Kojic Acid Derivative Synthesis in Polar Aprotic Solvent Using Lipozyme RMIM and Its Rheological Study

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Nurazwa IshakHelmi Wasoh

Abstract

The synthesis of kojic acid derivative (KAD) from kojic and palmitic acid (C16:0) in the presence of immobilized lipase from Rhizomucor miehei (commercially known as Lipozyme RMIM), was studied using a shake flask system. Kojic acid is a polyfunctional heterocycles that acts as a source of nucleophile in this reaction allowing the formation of a lipophilic KAD. In this study, the source of biocatalyst, Lipozyme RMIM, was derived from the lipase of Rhizomucor miehei immobilized on weak anion exchange macro-porous Duolite ES 562 by the adsorption technique. The effects of solvents, enzyme loading, reaction temperature, and substrate molar ratio on the reaction rate were investigated. In one-factor-at-a-time (OFAT) experiments, a high reaction rate (30.6 × 10-3 M·min-1) of KAD synthesis was recorded using acetone, enzyme loading of 1.25% (w/v), reaction time of 12 h, temperature of 50 °C and substrate molar ratio of 5:1. Thereafter, a yield of KAD synthesis was optimized via the response surface methodology (RSM) whereby the optimized molar ratio (fatty acid: kojic acid), enzyme loading, reaction temperature and reaction time were 6.74, 1.97% (w/v), 45.9 °C, and 20 h respectively, giving a high yield of KAD (64.47%). This conditio...Continue Reading

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Citations

Mar 14, 2019·Journal of Cosmetic Dermatology·Ahmad Firdaus B Lajis, Arbakariya B Ariff
May 6, 2020·Bioprocess and Biosystems Engineering·Prerana D Tomke, Virendra K Rathod
Dec 8, 2021·World Journal of Microbiology & Biotechnology·Bartłomiej ZieniukAgata Fabiszewska

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Methods Mentioned

BETA
Differential Scanning Calorimetry

Software Mentioned

Sigmaplot
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