Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus.

FEBS Letters
Ming-Liang HeHsiang-fu Kung

Abstract

SARS-associated coronavirus was identified as the etiological agent of severe acute respiratory syndrome and a large virus pool was identified in wild animals. Virus generates drug resistance through fast mutagenesis and escapes antiviral treatment. siRNAs targeting different genes would be an alternative for overcoming drug resistance. Here, we report effective siRNAs targeting structural genes (i.e., spike, envelope, membrane, and nucleocapsid) and their antiviral kinetics. We also showed the synergistic effects of two siRNAs targeting different functional genes at a very low dose. Our findings may pave a way to develop cost effective siRNA agents for antiviral therapy in the future.

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Citations

May 2, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C-Y LuL-M Huang
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Datasets Mentioned

BETA
AY304495

Methods Mentioned

BETA
transfection
PCR

Software Mentioned

BLAST
SARSi
R

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