Kinetics and thermodynamics of association of a phospholipid derivative with lipid bilayers in liquid-disordered and liquid-ordered phases

Biophysical Journal
Magda S C AbreuWinchil L C Vaz

Abstract

We have measured the rates of insertion into, desorption from, and spontaneous interlayer translocation (flip-flop) in liquid-disordered and liquid-ordered phase lipid bilayer membranes, of the fluorescent phospholipid derivative NBD-dimyristoylphosphatidyl ethanolamine. This study made use of a recently described method that exploits a detailed knowledge of the binding kinetics of an amphiphile to bovine serum albumin, to recover the insertion and desorption rate constants when the albumin-bound amphiphile is transferred through the aqueous phase to the membrane and vice versa. The lipid bilayers, studied as large unilamellar vesicles, were prepared from pure 1-palmitoyl-2-oleoylphosphatidylcholine in the liquid-disordered phase; and from two cholesterol-containing binary lipid mixtures, 1-palmitoyl-2-oleoylphosphatidylcholine and cholesterol (molar ratio of 1:1), and egg sphingomyelin and cholesterol (molar ratio of 6:4), both in the liquid-ordered phase. Insertion, desorption, and translocation rate constants and equilibrium constants for association of the amphiphile monomer with the lipid bilayers were directly measured between 15 degrees and 35 degrees C, and the standard free energies, enthalpies, and entropies, as well ...Continue Reading

References

Dec 11, 1992·Biochimica Et Biophysica Acta·R E Brown
Dec 24, 1991·Biochemistry·J C McIntyre, R G Sleight
Nov 27, 1990·Biochemistry·M B Sankaram, T E Thompson
Feb 18, 1988·Biochimica Et Biophysica Acta·R Homan, H J Pownall
May 12, 1981·Biochemistry·J W Nichols, R E Pagano
Jun 5, 1997·Nature·K Simons, E Ikonen
Jul 17, 1998·Biochimica Et Biophysica Acta·R Koynova, M Caffrey
Jan 19, 1999·Annual Review of Cell and Developmental Biology·D A Brown, E London
Jan 5, 2000·Biophysical Journal·A PokornyW L Vaz
Aug 15, 2002·Biochemical and Biophysical Research Communications·Luís M B B EstroncaWinchil L C Vaz
Jan 14, 2003·Biophysical Journal·Magda S C AbreuWinchil L C Vaz
Sep 26, 2003·Biophysical Journal·Rodrigo F M de AlmeidaManuel Prieto
May 14, 2004·Annual Review of Biophysics and Biomolecular Structure·Kai Simons, Winchil L C Vaz

❮ Previous
Next ❯

Citations

Mar 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Itay Budin, Jack W Szostak
Dec 3, 2008·The Journal of Chemical Physics·Sanoop RamachandranMohamed Laradji
Jan 22, 2008·The Journal of Chemical Physics·Joel D RevaleeP B Sunil Kumar
Dec 27, 2005·FEBS Letters·Gerrit van MeerMaarten R Egmond
Nov 21, 2009·Analytical Biochemistry·Maria João MorenoAdrian Velazquez-Campoy
Aug 5, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Filipe M Coreta-GomesMaria João Moreno
Jan 3, 2007·Biochimica Et Biophysica Acta·Shelley M Elvington, J Wylie Nichols
Jul 30, 2008·Bioelectrochemistry·Victor Agmo Hernández, Fritz Scholz
Sep 4, 2007·Biophysical Journal·Luís M B B EstroncaWinchil L C Vaz
Oct 7, 2004·Biophysical Journal·Luís M B B EstroncaWinchil L C Vaz
Mar 25, 2008·Biochimica Et Biophysica Acta·P A T MartinsM J Moreno
Jan 27, 2015·Nature Chemical Biology·Jannik Bruun LarsenDimitrios Stamou
Jun 21, 2016·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Ying ZhangHongtao Yu
Jun 19, 2007·The Journal of Biological Chemistry·Abhinav NathWilliam M Atkins
Apr 9, 2014·Journal of Lipid Research·Luís M B B EstroncaWinchil L C Vaz
Jun 13, 2015·Physical Chemistry Chemical Physics : PCCP·Hugo A L FilipeLuís M S Loura
Dec 22, 2017·The Journal of Membrane Biology·Filipe M Coreta-GomesMaria J Moreno
Jun 15, 2018·Nature Communications·Ranajay SahaIrene A Chen
Jul 2, 2018·The Journal of Chemical Physics·Moynul HasanMasahito Yamazaki
Dec 18, 2019·Biochimica Et Biophysica Acta. Biomembranes·Renato M S CardosoMaria João Moreno
Dec 23, 2009·ACS Nano·Alexey V TitovRyan Pearson
Nov 26, 2010·The Journal of Physical Chemistry. B·R M S CardosoM J Moreno
Dec 14, 2011·The Journal of Physical Chemistry. B·Joana ValérioEurico Melo
Oct 18, 2017·ACS Omega·Jaime SameloMaria João Moreno
Oct 15, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Birgit PlochbergerGerhard J Schütz

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.