Kinetics and thermodynamics of copper(II) binding to apoazurin

Journal of the American Chemical Society
Cheryl A Blasie, Jeremy M Berg

Abstract

The binding of copper(II) to apoazurin has been probed by isothermal titration calorimetry in cholamine buffer at pH 7.0. The standard enthalpy change was determined to be -10.0 +/- 1.4 kcal/mol. Each calorimetric trace reveals an initial exothermic phase followed by an endothermic phase. The calorimetric data could be fit to a kinetic model involving a bimolecular combination of copper(II) and apoazurin in an exothermic process (k = 2 +/-1 x 103 M-1 s-1, DeltaH degrees = -19 +/- 3 kcal/mol) to form an intermediate that spontaneously converts to Cu(II)-azurin in an endothermic process (k = 0.024 +/- 0.01 s-1, DeltaH degrees = +9 +/- 3 kcal/mol). These data suggest that copper(II) first combines with apoazurin in an irreversible process to form an intermediate that converts to copper(II)-azurin in a process driven by the release of water. The overall standard free energy of copper(II) binding to apoazurin is estimated to be -18.8 kcal/mol.

Citations

Aug 23, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Luc GuilloreauPeter Faller
Sep 17, 2005·FEBS Letters·Luis A Alcaraz, Antonio Donaire
Feb 9, 2005·Biochimica Et Biophysica Acta·B K Muralidhara, Pernilla Wittung-Stafshede
May 30, 2006·Biochimica Et Biophysica Acta·Hironao WakabayashiPhilip J Fay
Feb 21, 2006·Journal of Inorganic Biochemistry·Laura S Busenlehner, David P Giedroc
Feb 6, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Jessica MarksPernilla Wittung-Stafshede
Aug 23, 2019·Journal of the American Chemical Society·Molly L North, Dean E Wilcox
Dec 8, 2004·Inorganic Chemistry·Pernilla Wittung-Stafshede

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