PMID: 3756187Oct 17, 1986Paper

Kinetics of actin depolymerization: influence of ions, temperature, age of F-actin, cytochalasin B and phalloidin

Biochimica Et Biophysica Acta
H Wendel, P Dancker

Abstract

Actin, labelled with the fluorescent dye N-(3-pyrenyl)maleimide, was diluted below its critical concentration and depolymerization was followed by measuring the declining fluorescence intensity. The time courses of depolymerization were fitted to a sum of three exponentials. In most cases there was a fast initial phase followed by one or three slower ones. Increasing MgCl2 concentration slowed down depolymerization velocity, as did substitution of Tris-maleate buffer by phosphate buffer. Older F-actin preparations depolymerized more slowly than younger ones. Phalloidin strongly decreased depolymerization velocity even after sonication. In the presence of cytochalasin B depolymerization was more uniformly exponential than in the absence of cytochalasin B; overall depolymerization velocity was decreased by cytochalasin B. The results are discussed on the assumption that depolymerization kinetics reflect the length distribution of actin filaments during depolymerization.

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Citations

Jul 15, 2015·Biophysical Journal·Alexander Fuhrmann, Adam J Engler
Aug 17, 2011·Biophysical Journal·Kurt M SchmollerAndreas R Bausch
Sep 25, 2017·Scientific Reports·Esa-Pekka KumpulaInari Kursula
Mar 1, 1992·The American Journal of Physiology·S BurlacuJ Borejdo
Jul 30, 1997·Biochemical and Biophysical Research Communications·H BombardierC Gicquaud
Sep 24, 1987·Biochimica Et Biophysica Acta·H Wendel, P Dancker
Sep 24, 1987·Biochimica Et Biophysica Acta·H Wendel, P Dancker
Jul 9, 2004·Biochemical and Biophysical Research Communications·Shin-ya SaitoYasushi Ohizumi
Feb 15, 1989·Archives of Biochemistry and Biophysics·E Urbanik, B R Ware
Aug 19, 2021·Nature Communications·Julea VlassakisAmy E Herr

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