PMID: 6967731Jul 8, 1980Paper

Kinetics of activation of prekallikrein by prekallikrein activator

Biochemistry
D L TankersleyD D Schroeder

Abstract

A 28 000 molecular weight activator of prekallikrein was isolated from human plasma, and the kinetics of its enzymic activity toward prekallikrein was investigated. The activation follows Michaelis-Menten kinetics with a kcat of approximately 3 S(-1); Km is strongly dependent upon the ionic strength. Under suitable conditions the activation obeys first-order kinetics, and the first-order rate constant may be used to quantitate prekallikrein activator activity. Thus a two-stage assay, in which the first step involves the activation of prekallikrein by the activator and the second step quantitates the kallikrein generated, was developed to allow the measurement of prekallikrein activator in biologic samples. The prekallikrein activator content of therapeutic protein solutions, as determined by means of this assay, correlated well with the hypotensive activity of these solutions as determined in an animal model.

References

Jul 13, 1978·The New England Journal of Medicine·B M AlvingJ S Finlayson
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R J MandleA P Kaplan
Sep 1, 1974·The Journal of Clinical Investigation·S D RevakT E Hugli
Dec 1, 1974·The Journal of Clinical Investigation·C R McMillinA G Walton
Nov 1, 1974·Archives of Biochemistry and Biophysics·C SampaioE Shaw
Jan 1, 1973·Immunological Communications·A BagdasarianR W Colman
Apr 1, 1955·Biochimica Et Biophysica Acta·G W SCHWERT, Y TAKENAKA

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Citations

Sep 16, 1985·European Journal of Biochemistry·J RosingJ H Griffin
Oct 16, 2009·Biologicals : Journal of the International Association of Biological Standardization·Thanae GeorgakopoulosTimothy K Hayes
Feb 15, 1982·Thrombosis Research·D L TankersleyJ S Finlayson

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