Kinetics of ADP-induced human platelet shape change: apparent positive cooperativity

Canadian Journal of Physiology and Pharmacology
J G MiltonM M Frojmovic

Abstract

The kinetics of ADP-induced human platelet shape change have been examined. Initial velocities of platelet shape change were estimated by two methods: (1) the slope of the initial decrease in light transmission through stirred, citrated platelet-rich plasma, and (2) direct examination of platelet morphologies by phase-contrast microscopy. In both cases, a value of the Hill coefficient, NH, significantly greater than 1 is obtained (2.0 +/- 0.2 and 1.8 +/- 0.2, respectively). The observed elevated value of NH is not due to a substantial fraction of the ADP being platelet bound, the presence of factors in the plasma, platelet heterogeneity, or the influence of the rate of platelet shape change reversion. Our observations suggest that ADP-induced platelet shape change may be a positively cooperative or "threshold" type response.

Citations

Mar 1, 1986·The Journal of Clinical Investigation·E KorneckiR H Lenox
Sep 1, 1987·Thrombosis Research·J G Milton, M M Frojmovic
Jan 15, 1989·Thrombosis Research·D O BeaumontC W Slattery
Feb 1, 1982·British Journal of Haematology·K M ZawilskaN A Begent
May 10, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·János FentSusan Lakatos
Nov 8, 2001·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Z WangC Ruan
Feb 1, 1984·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·R LeblancM M Frojmovic
Jul 30, 1985·Biochimica Et Biophysica Acta·R R Hantgan

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