Kinetics of allergen expression in cultures of house dust mites, Dermatophagoides pteronyssinus and D. farinae (Acari: Pyroglyphidae)

Journal of Medical Entomology
E ErasoR Cisterna

Abstract

Laboratory cultures of house dust mites Dermatophagoides pteronyssinus (Trouessart, 1897) and Dermatophagoides farinae Hughes, 1961 were used to study the population dynamics of the mites and the kinetics of antigen appearance. The analysis of extracts obtained after different incubation periods, carried out by SDS-PAGE, immunoblotting, and enzyme-linked immunosorbent assay, allows for the definition of 3 different growth phases: the latency phase (F1); the exponential growth phase (F2) during which the allergenic proteins, including the Der 1 and Der 2 major allergens, were expressed more intensely and in larger quantities; and a final phase (F3), death, in which the lowest rates of allergenic components with a clearly different pattern were seen. The data obtained from this work demonstrates that mite cultures during the maximum growth phase (F2) contain the largest amount of allergenic components as well as the highest major allergen concentrations.

Citations

Aug 8, 2014·Experimental & Applied Acarology·Tomas Erban, Jan Hubert
Jan 13, 2016·Journal of Proteome Research·Jintarat ChoopongAnchalee Tungtrongchitr
Jan 22, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·W R Thomas
Sep 15, 2010·Experimental & Applied Acarology·Lakshmi YellaLarry G Arlian
Nov 23, 2018·Microbial Ecology·Jan HubertPavel Klimov
Sep 28, 2020·Experimental Parasitology·DongLing NiuWanYu Zhang

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