Kinetics of binding of oligosaccharides to a homogeneous pneumococcal antibody: dependence on antigen chain length suggests a labile intermediate complex

Biochemistry
H MaedaJ C Jaton

Abstract

Temperature-jump experiments were performed with di-, tetra-, and hexasaccharides derived from type III pneumococcal polysaccharide using a homogeneous corresponding antibody IgG 45-394. A decrease in stability of the oligosaccharide-antibody complexes with decreasing chain length was observed and entirely reflected in the decrease of the association rate constants which were 1.7 X 10(4) M-1 s-1 for the di-, 3.7 X 10(5) M-1 s-1 for the tetra-, and 1.1 X 10(6) M-1 s-1 for the hexasaccharide at 23 degrees C. The dissociation rate constants for all oligomers were about 12 s-1. This marked chain-length dependence of the association rate constants as well as their low values are unexpected for a single binding step. A mechanism is proposed which consists of a fast formation of a labile oligosaccharide-antibody precomplex followed by a slow isomerization step which is induced by the oligosaccharide ligands but which is chain-length independent.

References

Nov 5, 1981·Journal of Molecular Biology·P M ColmanW G Laver
Dec 1, 1986·Clinical Immunology and Immunopathology·M E CroninR W Lightfoot
Mar 31, 2018·European Biophysics Journal : EBJ·I Pecht
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·W M Kalka-MollD L Kasper

Citations

Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J SchlessingerI Pecht
Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D Lancet, I Pecht
Nov 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·D A HolowkaR E Cathou
Apr 16, 1971·Biochemical and Biophysical Research Communications·S A LevisonW B Dandliker
Mar 1, 1968·Immunochemistry·W B Dandliker, S A Levison

Related Concepts

Thermodynamics
Structure-Activity Relationship
Binding Sites, Antibody
Complex (molecular entity)
IgG2B
Triportheus paranensis
Plasma Protein Binding Capacity
Polysaccharides, Bacterial
Antigens
Polyvalent pneumococcal vaccine

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.