PMID: 3756176Sep 26, 1986Paper

Kinetics of Ca2+ binding to calmodulin and its tryptic fragments studied by 43Ca-NMR

Biochimica Et Biophysica Acta
A TelemanS Forsen

Abstract

The kinetics of calcium ion binding to bovine testis calmodulin and its tryptic fragments have been studied by 43Ca-NMR. The same subdivision of the Ca2+-binding sites of calmodulin into two with slow exchange (high affinity) and two with fast exchange (low affinity) observed at low ionic strength is also encountered at high ionic strength. The effect of 0.1 M KCl is to reduce the exchange rate of the fast process from 1150 s-1 to 520 s-1 at 25 degrees C. Studies of the tryptic fragments TR1C and TR2C, comprising the N- or C-terminal half of calmodulin, respectively, clearly identified Ca2+-binding domains I and II as those with fast exchange (low affinity) and domains III and IV as those with slow exchange (high affinity). Activation parameters are reported for calmodulin and TR1C. Correlation times have been measured for ions bound to the fragments. The obtained values, 5 and 6 ns for TR1C and TR2C, respectively, are of the same order as rotational correlation times for the entire fragment molecules, indicating that the calcium ions do not have any mobility with a correlation time in the ns range within the sites.

References

Jul 15, 1985·European Journal of Biochemistry·R J Williams
May 2, 1985·Nature·Y S BabuW J Cook
Jul 16, 1985·Biochemical and Biophysical Research Communications·C L Wang
Jul 20, 1981·FEBS Letters·J KuznickiP Cohen
May 1, 1983·Annals of Internal Medicine·H Rasmussen
Aug 15, 1983·European Journal of Biochemistry·O TelemanE Thulin
Jan 13, 1984·Biochemical and Biophysical Research Communications·A AulabaughW A Gibbons
Jan 16, 1984·European Journal of Biochemistry·D C DalgarnoW Drabikowski
Feb 15, 1984·European Journal of Biochemistry·R E KlevitR J Williams
Apr 16, 1984·Biochemical and Biophysical Research Communications·P BayleyS Forsen
Jan 1, 1982·Advances in Protein Chemistry·C B Klee, T C Vanaman

❮ Previous
Next ❯

Citations

Jun 15, 1994·Molecular and Cellular Biochemistry·M P Walsh
May 6, 1999·Bioelectrochemistry and Bioenergetics·A A PillaB F Sisken
Jan 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hye Yoon ParkLois Pollack
Aug 24, 2010·The Journal of Biological Chemistry·Zhiping P PangThomas C Südhof
Oct 15, 1988·European Journal of Biochemistry·S ForsénT Grundström
Nov 13, 1990·European Journal of Biochemistry·D PietrobonT Pozzan
Jul 20, 2000·Journal of Molecular and Cellular Cardiology·N FinleyP R Rosevear
Oct 18, 2011·Biochimica Et Biophysica Acta·Guido C Faas, Istvan Mody
Aug 1, 1994·Quarterly Reviews of Biophysics·J J FalkeO B Peersen
Feb 1, 1987·Quarterly Reviews of Biophysics·S ForsénH Wennerström
Oct 24, 1998·The Journal of Biological Chemistry·A MalmendalS Forsén
Nov 25, 2018·The Journal of General Physiology·Worawan B LimpitikulRaimond L Winslow
Feb 26, 2020·International Journal of Molecular Sciences·Walter J Chazin, Christopher N Johnson
Oct 8, 1999·Biochemistry·A MalmendalT Drakenberg
Jul 27, 2021·Frontiers in Cell and Developmental Biology·Sunday OkutachiDaniel Abankwa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.