Kinetics of cellular proliferation in regenerating mouse liver pretreated with the alkylating drug dipin

Virchows Archiv. B, Cell Pathology Including Molecular Pathology
V M FaktorW Y Brodsky

Abstract

The effect of prior exposure to the alkylating drug 1,4-Bis[N,N'-di(ethylene)-phosphamide] piperazine (Dipin) on the cell cycle progression in a regenerating mouse liver is reported, using cytological, autoradiographical and DNA-cytophotometrical methods. In Dipin-treated animals 3H-TdR pulse labelled nuclei appeared 22 h after the partial hepatectomy, followed by a rapid rise in labelling index to a very high level between 43-54 h, and then by a gradual decline to 72 h. Four injections of 3H-thymidine at 16 h intervals resulted in 89% labelling of hepatocytes at 72 h. The DNA-synthesis time was assessed by DNA photometry combined with 3H-TdR autoradiography on the same nucleus. The DNA-content in Dipin-pretreated nuclei was doubled after 26 h, approximately three times longer than in the controls. Mitoses were completely absent during the first 2-4 days. They were observed subsequently for up to 2 months but with varying frequency. The frequency of binucleated hepatocytes remained constant during liver regeneration. Hence, pretreatment of resting liver with Dipin altered the kinetics of cell proliferation following partial hepatectomy. Delay in division resulted primarily from an increase in the DNA-synthesis time and a prolon...Continue Reading

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Citations

May 1, 1990·Biulleten' eksperimental'noĭ biologii i meditsiny·S A Radaeva (Pronina), V M Faktor
Sep 1, 1996·Biulleten' eksperimental'noĭ biologii i meditsiny·I V UryvaevaG V Delone
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·E P BöttingerM B Sporn
Aug 8, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M R JensenS S Thorgeirsson
Aug 1, 1993·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·V M Factor, S A Radaeva
Oct 1, 1990·Differentiation; Research in Biological Diversity·N V EngelhardtM N Lasareva
Aug 10, 2000·The American Journal of Pathology·K M Braun, E P Sandgren
Apr 2, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Michael Rugaard JensenSnorri S Thorgeirsson

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