PMID: 2110473Mar 13, 1990Paper

Kinetics of coagulation factor X activation by platelet-bound factor IXa

Biochemistry
R Rawala-SheikhP N Walsh

Abstract

Thrombin-activated human platelets, in the presence of factors VIIIa and X, have specific, high-affinity (Kd approximately 0.5 nM), saturable binding sites for factor IXa that are involved in factor X activation [Ahmad, S.S., Rawala-Sheikh, R., & Walsh, P.N. (1989) J. Biol. Chem. 264, 3244-3251]. To determine the functional consequences of factor IXa binding to platelets, a detailed kinetic analysis of the effects of platelets, phospholipids, and factor VIII on factor IXa catalyzed factor X activation was done. In the absence of platelets, phospholipids, or factor VIII, the Michaelis constant (Km = 81 microM) was greater than 500-fold higher than the factor X concentration in human plasma. Unactivated platelets and thrombin-activated factor VIII, alone or in combination, had no effect on the kinetic parameters, whereas thrombin-activated platelets caused a major decrease in Km (0.39 microM) with no significant effect on kcat (0.052 min-1) and allowed factor VIIIa to decrease the Km further to a concentration (0.16 microM) near that of factor X in plasma and to increase the kcat 24,000-fold to 1240 min-1. Sonicated mixed phosphatidylserine/phosphatidylcholine vesicles (25/75, mol/mol) had kinetic effects similar to those of acti...Continue Reading

References

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J P MiletichP W Majerus
Nov 14, 1978·Biochemistry·B Dahlbäck, J Stenflo
Jul 1, 1986·Biochemistry·J S GreengardJ H Griffin
Jun 1, 1972·British Journal of Haematology·P N Walsh, R Biggs
Jun 1, 1983·European Journal of Biochemistry·J van RijnG van Dieijen
Apr 1, 1982·The Journal of Clinical Investigation·M B Hultin

❮ Previous
Next ❯

Citations

Oct 1, 1994·American Journal of Hematology·P N Walsh
Nov 1, 1994·Trends in Cardiovascular Medicine·S S Ahmad, P N Walsh
Jun 30, 1998·Thrombosis Research·M A KhaninA E Kogan
Dec 20, 2003·Blood Reviews·Philip J Fay
Oct 31, 2002·British Journal of Haematology·Evgueni L SaenkoGeoffrey Kemball-Cook
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·S S AhmadP N Walsh
May 5, 2010·Mathematical Medicine and Biology : a Journal of the IMA·Karin Leiderman, Aaron L Fogelson
Jul 19, 2001·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·A Kramoroff, J M Nigretto
Sep 25, 2007·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Donghui Zhu
Oct 6, 2007·Blood·Ting-Chang HsuArthur R Thompson
Oct 14, 2010·PLoS Computational Biology·Manash S ChatterjeeScott L Diamond
Aug 27, 2013·Journal of Theoretical Biology·Evgeny A ShavlyuginMikhail A Khanin
Sep 9, 2009·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Offer ErezRoberto Romero
May 1, 1997·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·M S KimA G Greenburg
Feb 20, 2008·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Alisa S Wolberg, Robert A Campbell
Oct 1, 2004·Journal of Thrombosis and Haemostasis : JTH·D A GabrielH R Roberts
Oct 1, 2004·Journal of Thrombosis and Haemostasis : JTH·J E PhillipsG E Gilbert
May 12, 2010·Journal of Thrombosis and Haemostasis : JTH·M F WhelihanK G Mann
Oct 1, 1992·Journal of Comparative Pathology·P A Gentry
Jan 9, 2007·Blood Reviews·Alisa S Wolberg
Oct 31, 2006·Mathematical Biosciences·Kirill V Tyurin, Mikhail A Khanin
Dec 4, 2012·Biophysical Journal·N M DashkevichF I Ataullakhanov
Jun 20, 1998·The Journal of Biological Chemistry·D H HoP N Walsh
Mar 25, 2004·The Journal of Biological Chemistry·Saulius ButenasKenneth G Mann
Jul 22, 2004·The Journal of Biological Chemistry·Randolf J KerschbaumerFriedrich Scheiflinger
Aug 4, 2018·Biomechanics and Modeling in Mechanobiology·John D HornJason M Ortega
Jul 15, 2015·Annals of Biomedical Engineering·E Victoria Dydek, Elliot L Chaikof
Jul 21, 1998·The Journal of Biological Chemistry·P J Fay, K Koshibu
Dec 1, 2001·The Journal of Biological Chemistry·Frank H WilkinsonPeter N Walsh
Oct 6, 1995·The Journal of Biological Chemistry·F MahdiA H Schmaier
Apr 25, 2000·The Journal of Biological Chemistry·S S AhmadP N Walsh
Mar 11, 2004·The Journal of Biological Chemistry·Fredda S LondonPeter N Walsh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.