Kinetics of corneal leukocytes by intravital multiphoton microscopy

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Yashar Seyed-RazaviPedram Hamrah

Abstract

Corneal immune privilege is integral in maintaining the clear avascular window to the foreign world. The presence of distinct populations of corneal leukocytes (CLs) in the normal cornea has been firmly established. However, their precise function and kinetics remain, as of yet, unclear. Through intravital multiphoton microscopy (IV-MPM), allowing the means to accumulate critical spatial and temporal cellular information, we provide details for long-term investigation of CL morphology and kinetics under steady state and following inflammation. Significant alterations in size and morphology of corneal CD11c+ dendritic cells (DCs) were noted following acute sterile inflammation, including cell volume (4364.4 ± 489.6 vs. 1787.6 ± 111.0 μm3, P < 0.001) and sphericity (0.82 ± 0.01 vs. 0.42 ± 0.02, P < 0.001) compared with steady state. Furthermore, IV-MPM analyses revealed alterations in both the CD11c+ DC and major histocompatibility complex class II (MHC)-II+ mature antigen-presenting cell population kinetics during inflammation, including track displacement length (CD11c: 16.57 ± 1.41 vs. 4.64 ± 0.56 μm, P < 0.001; MHC-II: 9.03 ± 0.37 vs. 4.09 ± 0.39, P < 0.001) and velocity (CD11c: 1.91 ± 0.07 μm/min vs. 1.73 ± 0.1302 μm/min; MH...Continue Reading

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Citations

Aug 21, 2019·Ocular Immunology and Inflammation·Haihan JiaoHolly R Chinnery
May 1, 2020·BioMed Research International·Holly R Chinnery, Kate E Keller
Jul 28, 2020·Progress in Retinal and Eye Research·Arsia JamaliPedram Hamrah
Jul 10, 2021·Clinical & Experimental Ophthalmology·Holly R ChinneryLaura E Downie

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