Kinetics of cyanide and carbon monoxide dissociation from ferrous human haptoglobin:hemoglobin(II) complexes.

Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
Paolo AscenziMassimo Coletta

Abstract

Haptoglobin (Hp) counterbalances the adverse effects of extra-erythrocytic hemoglobin (Hb) trapping the αβ dimers of Hb. In turn, the Hp:Hb complexes display heme-based reactivity. Here, the kinetics of cyanide and carbon monoxide dissociation from ferrous-ligated Hp:Hb complexes are reported at pH 7.0 and 20.0 °C. Cyanide dissociation from Hp1-1:Hb(II)-CN- and Hp2-2:Hb-CN- has been followed upon the dithionite-mediated conversion of ferric to ferrous-ligated Hp:Hb complexes. Values of kon for the dithionite-mediated reduction of Hp1-1:Hb(III)-CN- and Hp2-2:Hb(III)-CN- are (7.3 ± 1.1) × 106 M-1 s-1 and (6.2 ± 1.0) × 106 M-1 s-1, respectively. Values of the first-order rate constant (i.e., h) for cyanide dissociation from Hp1-1:Hb(II)-CN- and Hp2-2:Hb(II)-CN- are (1.2 ± 0.2) × 10-1 s-1 and (1.3 ± 0.2) × 10-1 s-1, respectively. CO dissociation from Hp:Hb(II)-CO complexes has been followed by replacing CO with NO. Values of the first-order rate constant (i.e., l) for CO dissociation from Hp1-1:Hb(II)-CO are (1.4 ± 0.2) × 10-2 s-1 and (6.2 ± 0.8) × 10-3 s-1, and those from Hp2-2:Hb(II)-CO are (1.3 ± 0.2) × 10-2 s-1 and (7.3 ± 0.9) × 10-3 s-1. Values of kon, h, and l correspond to those reported for the R-state of tetrameric Hb and ...Continue Reading

References

Nov 1, 1977·The Biochemical Journal·R P Cox
Jan 1, 1992·Protein Engineering·F C Wireko, D J Abraham
Jan 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·Q H Gibson
May 1, 1973·The Biochemical Journal·E ChianconeF Lavialle
Dec 28, 1966·Journal of Molecular Biology·R L Nagel, Q H Gibson
Apr 5, 1984·Journal of Molecular Biology·J C WejmanJ Greer
Apr 5, 1984·Journal of Molecular Biology·J C WejmanJ Greer
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A KuroskyW M Fitch
Apr 13, 1994·Biochimica Et Biophysica Acta·G AntoniniM Brunori
Jan 1, 1996·Annual Review of Biochemistry·H B Gray, J R Winkler
Jan 1, 1997·Progress in Biophysics and Molecular Biology·M BolognesiP Ascenzi
Feb 24, 2001·Nature·M KristiansenS K Moestrup
Apr 12, 1965·Biochimica Et Biophysica Acta·R L NAGELH M RANNEY
May 5, 2004·Biochemistry·Mario MilaniPaolo Ascenzi
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Jun 13, 2006·Journal of Molecular Biology·Sam-Yong ParkJeremy R H Tame
Oct 4, 2007·Biochemical and Biophysical Research Communications·Paolo AscenziMassimo Coletta
Mar 28, 2008·Nitric Oxide : Biology and Chemistry·Ivan AzarovDaniel B Kim-Shapiro
Oct 31, 2008·The FEBS Journal·F PolticelliP Ascenzi
Apr 15, 2009·Biopolymers·Carmelinda SavinoBeatrice Vallone
Aug 28, 2012·Nature·Christian Brix Folsted AndersenSøren Kragh Moestrup
Nov 13, 2012·Trends in Biotechnology·Abdu I AlayashLeif Bülow
Feb 4, 2014·Free Radical Biology & Medicine·Todd L MollanAbdu I Alayash
Nov 21, 2014·Nature Communications·Kristian StødkildeChristian B F Andersen
May 28, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Paolo AscenziMassimo Coletta
Sep 22, 2016·Antioxidants & Redox Signaling·Christian Brix Folsted AndersenSøren Kragh Moestrup
Apr 2, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Paolo AscenziMassimo Coletta
Jul 25, 2018·The Journal of Physical Chemistry. B·Paolo Ascenzi, Massimo Coletta
Aug 3, 2018·Journal of Inorganic Biochemistry·Paolo AscenziMassimo Coletta
Feb 2, 2019·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Paolo AscenziMassimo Coletta
Nov 17, 2019·Journal of Inorganic Biochemistry·Paolo AscenziMassimo Coletta

❮ Previous
Next ❯

Citations

Jul 15, 2020·Molecular Aspects of Medicine·Alessandra di MasiPaolo Ascenzi

❮ Previous
Next ❯

Software Mentioned

Chimera Package
UCSF
GraphPad Prism
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.