Kinetics of DNA methylation inheritance by the Dnmt1-including complexes during the cell cycle.

Cell Division
Eric HervouetPierre-François Cartron

Abstract

The clonal transmission of lineage-specific DNA methylation patterns in a mammalian genome during the cellular division is a crucial biological process controlled by the DNA methyltransferase Dnmt1, mainly. To investigate possible dynamic mechanisms of DNA methylation inheritance during the cell cycle, we used a Proximity Ligation In Situ Assay (P-LISA) to analyze the kinetic of formation and DNA recruitment of Dnmt1-including complexes. P-LISA, sequential chromatin immunoprecipitation and quantitative methylation specific PCR revealed that the Dnmt1/PCNA/UHRF1-including complexes are mainly formed and recruited on DNA during the S-phase of cell cycle, while the formation and the DNA recruitment of several Dnmt1/transcription factors-including complexes are not S-phase dependent but are G0/G1 and/or G2/M phases dependent. Our data confirm that DNA methylation inheritance occurs in S-phase, and demonstrate that DNA methylation inheritance can also occur in G0/G1 and G2/M phases of the cell cycle.

References

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May 1, 2010·Genes & Cancer·Eric HervouetPierre-François Cartron

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Citations

Dec 10, 2015·Scientific Reports·Amy R VandiverKasper D Hansen
Apr 7, 2016·European Journal of Medicinal Chemistry·Vassilios MyrianthopoulosEmmanuel Mikros
Jan 8, 2015·Frontiers in Immunology·Pilar M Dominguez, Rita Shaknovich
Jun 9, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Maria S JensenRosa L López-Marqués
Dec 23, 2014·Epigenetics : Official Journal of the DNA Methylation Society·Cécile DesjobertJoëlle Riond
Jan 1, 2013·Cells·Eric HervouetPierre-François Cartron
Feb 17, 2018·Clinical Epigenetics·Eric HervouetPierre-François Cartron
Sep 23, 2016·G3 : Genes - Genomes - Genetics·Matthew V CannonDavid Serre
Jul 3, 2020·Bulletin of Mathematical Biology·Kiersten Utsey, James P Keener
Aug 1, 2021·Nucleic Acids Research·Miriam RecaldeCarmen Berasain

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Methods Mentioned

BETA
Assay
immunoprecipitation
PCR
PCRs
ChIP
Pull-Down
electrophoresis

Software Mentioned

Huygens Essential
BlobFinder
ImageJ
ApoTome
Amira

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