PMID: 11914118Mar 27, 2002Paper

Kinetics of early in vitro development of bovine in vivo- and in vitro-derived zygotes produced and/or cultured in chemically defined or serum-containing media

Reproduction : the Official Journal of the Society for the Study of Fertility
P HolmH Callesen

Abstract

The kinetics of the in vitro development of early embryos from bovine zygotes derived in vitro and in vitro were compared, investigating the effect of serum during in vitro maturation and fertilization (IVM-IVF) and in culture. Zygotes were collected from superovulated heifers or produced in vitro from immature oocytes with or without serum supplementation, and cultured subsequently in defined culture medium (SOFaaci) with or without serum supplementation. Time-lapse images were recorded every 0.5 h throughout the culture period. More in vivo- than in vitro-derived zygotes developed to the compact morula or blastocyst stages (87% versus 47-54%, respectively; P < 0.05). Embryo development was blocked predominantly at the second or fourth cell cycles (28 and 29%). However, blastomeres degenerated at all cleavage stages. Serum supplementation during IVM-IVF resulted in abnormally sized blastomeres at first cleavage (defined serum: 20-22% versus in vivo-derived: 8%, P < 0.05). The duration of the second, third and fifth cell cycles of in vivo-derived zygotes were 1-5 h shorter compared with those of in vitro-derived zygotes cultured under similar conditions (P < 0.05). However, the kinetics of embryo development was affected by ser...Continue Reading

Citations

Oct 19, 2013·Reproductive Biomedicine Online·B AparicioM Meseguer
Feb 18, 2004·The Journal of Reproduction and Development·Hisataka IwataMasashi Miyake
Apr 23, 2014·Current Opinion in Obstetrics & Gynecology·Necati Findikli, Engin Oral
Dec 17, 2009·Reproduction, Fertility, and Development·Henrik Callesen
Dec 7, 2013·Reproduction, Fertility, and Development·M HoelkerD Tesfaye
May 8, 2008·Current Opinion in Obstetrics & Gynecology·Kevin S Richter
Feb 12, 2013·Fertility and Sterility·Javier Herrero, Marcos Meseguer
Dec 16, 2004·Reproduction in Domestic Animals = Zuchthygiene·G F MastromonacoW A King
Jul 31, 2003·Reproduction in Domestic Animals = Zuchthygiene·P LonerganM P Boland
Jul 26, 2006·Molecular Reproduction and Development·D Fischer RussellD H Betts
Mar 28, 2008·Reproduction in Domestic Animals = Zuchthygiene·U BesenfelderK Schellander
Jan 5, 2005·Theriogenology·Anne-Sophie LequarrePascal Mermillod
Sep 12, 2014·Reproduction, Fertility, and Development·Alfonso Gutierrez-AdanMellissa R W Mann
Dec 4, 2012·Theriogenology·Cesar A CastanedaMichael J D'Occhio

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