PMID: 42246Jan 1, 1979

Kinetics of elementary steps in the cytochrome P-450 reaction sequence. IV. Mechanism of the NADPH reduction reaction of cytochrome P-450LM

Acta biologica et medica Germanica
J BlanckK Ruckpaul

Abstract

The aerobic NADPH reduction of the Triton N-101 disintegrated cytochrome P-450LM system has been studied. At this organization level--the components being dispersed in solution--a first-order monophasic reaction is exhibited. Neither the complex formation of cytochrome and reductase, respectively, nor preceding diffusion is rate limiting. The initial rate follows the ratio reductase/P-450 (mole/mole) thus indicating a Michaelis-Menten type enzyme mechanism. A model treatment of the reaction fits the systems behaviour as a whole. A multiparameter equilibrium state and different specified time function equations were developed for the determination of individual step rate constants and other system parameters as well.

Related Concepts

Nevus
Talpidae
Aerobiosis
Cytochrome P450
Cytochrome P-450 Oxygenase
Hydatidiform Mole
Microsomes, Liver
Cytochromes
Triton N 101
NADP

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