PMID: 616067Oct 1, 1977Paper

Kinetics of ethanol inhibition of galactose elimination in perfused pig liver

Scandinavian Journal of Clinical and Laboratory Investigation
S KeidingK Tønnesen

Abstract

The effect of ethanol (5--25 mM) on the galactose elimination kinetics in the intact liver was studied in the isolated perfused pig liver, using the steady-state infusion technique. Ethanol reduced galactose-Vmax on average to 0.07 mmol/min kg liver in six experiments from 0.43 mmol/min kg obtained in control experiments without ethanol. Also Km was significantly reduced from 0.23 mmol/l plasma water to 0.03 mmol/l. Ethanol increased UDP-galactose ten-fold simultaneous with a rise in hepatic outflow ratio of lactate to pyruvate to about 300 from 10; this indicates that ethanol inhibits epimerase. In experiments with increasing galactose elimination rates, the concentration of galactose-1-P increased much less than the concentration of galactose, and the phosphorylation of galactose therefore seems to be rate-limiting. In vitro galactokinase is inhibited by galactose-1-P. In the present study ethanol increased galactose-1-P five to ten times, and the reduction of Vmax and Km by ethanol could be explained by uncompetitive inhibition by galactose-1-P with Ki about 0.1 mmol/l. Ethanol decreased UDP-glucose to about 40% and UTP to less than 5%, probably due to trapping as UDP-galactose. This may depress the forward transferase react...Continue Reading

References

Sep 21, 1976·Journal of Theoretical Biology·L BassN Tygstrup
May 1, 1976·The American Journal of Physiology·S KeidingN Tygstrup
May 1, 1973·Scandinavian Journal of Clinical and Laboratory Investigation·S Keiding
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Citations

Aug 3, 1999·Transplant International : Official Journal of the European Society for Organ Transplantation·C A SeilerJ Reichen
May 17, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michael SørensenSusanne Keiding
Jan 1, 1986·European Journal of Clinical Pharmacology·M BührerJ Bircher
Aug 19, 2007·Metabolism: Clinical and Experimental·Ton-Ho YoungOliver Yoa-Pu Hu
Nov 1, 1986·The Journal of Surgical Research·W J SchirmerD E Fry
Apr 7, 1983·Journal of Theoretical Biology·H VilstrupP B Vendsborg
Oct 9, 2008·Laboratory Animals·T H YoungO Y P Hu
Jan 1, 1981·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M P SalaspuroC S Lieber
Mar 15, 1983·Klinische Wochenschrift·M RössleW Gerok
Oct 1, 1979·The American Journal of Physiology·S KeidingL Christiansen

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