Kinetics of excited states of pigment clusters in solubilized light-harvesting complex II: photon density-dependent fluorescence yield and transmittance

Biophysical Journal
R SchödelG Renger

Abstract

Relative fluorescence yield, phi F, and transmittance, T, were measured in solubilized light-harvesting complex II (LHCII) as a function of photon density, Ip, of monochromatic 645-nm laser pulses (duration: approximately 2.5 ns). Special efforts were made in constructing an optical set-up that allows the accurate determination of the fluorescence from an area of constant Ip, phi F(Ip) starts to decline at approximately 10(14) and drops to values below 0.01% at maximum Ip (approximately 10(19) photons cm-2 pulse-1). T(Ip) decreases only slightly at photon densities of approximately 10(15) but increases steeply at values of > 10(17) photons cm-2 pulse-1. The interpretation of the phi F(Ip) data using the saturation limit of Mauzerall's multiple hit model leads to a unit size of about 10-15 chlorophyll molecules. One interpretation is to attribute this result to a very fast exciton-exciton annihilation of multiple excited states generated within this small domain. Alternatively, based on the assumption that delocalized cluster states within the monomeric/trimeric subunit of LHCII exist, the results can be consistently described by a kinetic model comprising ground, monoexcitonic, and biexcitonic states of clusters and a triplet s...Continue Reading

Citations

May 28, 2011·Biochimica Et Biophysica Acta·Petar H LambrevGernot Renger
Apr 28, 2001·Biophysical Journal·V BarzdaL Valkunas
Apr 17, 2013·Physical Chemistry Chemical Physics : PCCP·Maxim F GelinWolfgang Domcke
Jan 20, 2011·The Journal of Physical Chemistry Letters·Maxim F GelinWolfgang Domcke
Mar 24, 2011·The Journal of Physical Chemistry. B·Maxim F GelinWolfgang Domcke

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