Abstract
The purpose of the present study was to investigate the evolution of expression of mRNA message for the genes for the genome stress sensor GADD45α, the apoptosis initiator TP53 and the apoptosis executor CASP3 in the rat lens in vivo in response to exposure to UVR around 300 nm. Forty six week old female albino Sprague-Dawley rats were unilaterally exposed to double threshold dose for cataract induction, 8 kJ/m(2) (8.9 W/m(2) for 15 min), of UVR (λ(max) = 300 nm). The animals were sacrificed at 1, 5, 24 and 120 h following exposure to UVR-B. For each of the GADD45α, TP53 and CASP3 genes, respectively, mRNA expression in the lenses was measured by quantitative RT-PCR. It was found that expression of mRNA for GADD45α transiently increases between 5 and 24 h after exposure. TP53 is slightly downregulated in exposed lenses at 1 and 5 h after exposure and thereafter the mRNA expression increases with a constant rate of 9.4\ 10(-3) rel. units/h to a 1.8 fold increase at 120 h after exposure. Expression of mRNA for CASP3 is downregulated at 1, 5 and 24 h after in vivo exposure and then increases with a constant rate of 4.7 10(-3) rel. units/h, upto a 1.3 fold upregulation at 120 h. Double threshold dose of UVR, for short delay onset o...Continue Reading
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Citations
Apr 5, 2013·Current Eye Research·Martin KronschlägerPer G Söderberg
Apr 5, 2014·Acta Ophthalmologica·Nooshin TalebizadehPer Söderberg
Jun 5, 2016·Experimental Eye Research·Stefan Löfgren
Sep 23, 2014·PloS One·Nooshin TalebizadehPer Söderberg
Jan 14, 2017·Acta Ophthalmologica·Konstantin Galichanin
Sep 26, 2019·PloS One·Yuyan YouYanhui Liu
Mar 22, 2016·Experimental and Therapeutic Medicine·Yuchen DongMeisheng Zhao