Kinetics of hematopoiesis in Dexter-type long-term cultures established from human umbilical cord blood cells

Stem Cells
Hector MayaniO T Ramírez

Abstract

In the present study, we have established Dexter-type long-term cultures (D-LTC) from human umbilical cord blood (UCB) and followed the kinetics of different hematopoietic progenitor cells (HPCs)--including multipotent (colony forming unit [CFU]-Mixture), erythroid (CFU-erythroid, BFU-E), and myeloid (CFU-granulocyte, CFU-macrophage, CFU-granulocyte/marcophage) progenitors as well as of morphologically recognizable erythroid, myeloid and lymphoid cells--during a nine-week culture period. D-LTC were also established from adult bone marrow (BM) as controls. On day 0, both UCB and BM showed similar total numbers of HPCs (about 310/10(5) cells), however, UCB showed a higher proportion of primitive HPCs (i.e., CFU-Mixture, CFU-granulocyte/macrophage and BFU-E). A poor adherent cell layer, consisting almost exclusively of macrophages, was developed in UCB D-LTC and this correlated with a continuous decline in HPC numbers throughout the culture period. In contrast, adherent cell numbers in BM D-LTC, including fibroblasts and macrophages, were two- to fourfold higher than in UCB cultures, and the numbers of HPCs were also significantly higher, reaching plateau levels between weeks 6 and 9. In both types of cultures, erythroid and multi...Continue Reading

References

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Citations

Jan 22, 2004·Archives of Medical Research·Héctor MayaniPatricia Flores-Guzmán
Jul 17, 2001·Journal of Hematotherapy & Stem Cell Research·G Martínez-JaramilloH Mayani
Sep 10, 2010·Stem Cells and Development·Hera Andrade-ZaldívarAntonio De León-Rodríguez
Apr 1, 2009·Expert Review of Hematology·Phuong L Doan, Nelson J Chao
Dec 24, 2015·Frontiers in Medicine·Rohtesh S MehtaElizabeth J Shpall
Mar 1, 2008·British Journal of Haematology·Fernando O Pinto, Irene Roberts
Aug 11, 2006·Current Opinion in Biotechnology·Daniel C Kirouac, Peter W Zandstra
Feb 24, 2007·Differentiation; Research in Biological Diversity·Teodora NikolovaAnna M Wobus
Oct 26, 2005·The Journal of Laboratory and Clinical Medicine·Fei-Zhou LuXiao-Kang Li
Jul 21, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·N B Nardi, Z Z Alfonso
Feb 5, 2019·Cytotechnology·Ana K Gutiérrez-GarcíaAntonio De León-Rodríguez
Apr 25, 2012·World Journal of Gastrointestinal Surgery·Noemí Eiró, Francisco J Vizoso
Jun 6, 2018·Proceedings of the National Academy of Sciences of the United States of America·Paul E BourgineIvan Martin

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